| The Microdomain Forming Stomatin Family in the Ciliated Protozoan Paramecium tetraurelia: Identification, Localization and Functional Implications. | |
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MedLine Citation:
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PMID: 23376944 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
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The SPFH protein superfamily is assumed to occur universally in eukaryotes, but from protozoa information is scarce. In the Paramecium genome we found only Stomatins, 20 paralogs grouped in 8 families, STO1 to STO8. According to cDNA analysis all are expressed and molecular modeling shows the typical SPFH domain structure for all subgroups. For further analysis we used family-specific sequences for fluorescence and immuno-gold labeling, gene silencing, and functional tests. With all family members tested we found a patchy localization at/near the cell surface and on vesicles. The Sto1p and Sto4p families are also associated with the contractile vacuole complex, and Sto4p makes puncta on some food vacuoles and also is abundant on vesicles recycling from the release site of spent food vacuoles to the site of nascent food vacuole formation. Silencing of STO1 family reduces mechanosensitivity (ciliary reversal upon touching an obstacle), thus suggesting relevance for positioning of mechanosensitive channels in the plasmalemma. Silencing of STO4 members increases pulsation frequency of the contractile vacuole complex and reduces phagocytotic activity of Paramecium cells. In summary, Sto1p and Sto4p members seem to be involved in positioning specific superficial and intracellular microdomain-based membrane components whose functions may depend on mechanosensation (extracellular stimuli and internal osmotic pressure). |
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Authors:
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Alexander T Reuter; Claudia A O Stuermer; Helmut Plattner |
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Publication Detail:
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Type: JOURNAL ARTICLE Date: 2013-2-2 |
Journal Detail:
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Title: Eukaryotic cell Volume: - ISSN: 1535-9786 ISO Abbreviation: Eukaryotic Cell Publication Date: 2013 Feb |
Date Detail:
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Created Date: 2013-2-4 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 101130731 Medline TA: Eukaryot Cell Country: - |
Other Details:
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Languages: ENG Pagination: - Citation Subset: - |
Affiliation:
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Department of Biology, P.O.Box 5560, University of Konstanz, 78457 Konstanz, Germany. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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