| Microdissection and PCR-SSCP detected mutation and expression of p53 and K-ras gene in carcinogenesis and development of induced rat lung carcinoma | |
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MedLine Citation:
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PMID: 12417091 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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OBJECTIVE: To investigate the roles of p53 and K-ras gene in carcinogenesis and development of the lung carcinoma induced by 3-methylcholanthrene (MCA) and diethylinitrosamine (DEN) in Wistar rats, and to elucidate the relationships between the protein expression and gene mutation of p53 and K-ras. METHODS: Microdissection was used to obtain pure cell populations of each phase in the carcinogenesis and development of lung carcinoma induced by MCA and DEN. DNA of the microdissected cell populations was extracted and used to analyze the mutations of p53 exons 5 approximately 8 and K-ras exons 1 approximately 2 by PCR-SSCP. The expressions of p53 and K-ras protein in each phase were detected by immunohistochemistry. RESULTS: No mutation and protein expression of p53 and K-ras was found in the 30 cases with normal bronchial epithelium. Mutation of p53 was detected in 3.1% of 18 hyperplasia and 14 squamous metaplasia cases, 28.6% of 21 dysplasia, 30.0% of 12 carcinomas in situ, 51.2% of 43 infiltration carcinomas, 52.9% of 17 metastases. The positive immunostaining rate of p53 protein was 0, 42.9%, 50.0%, 60.5% and 64.7% respectively. K-ras mutation rate was 0, 4.8%, 8.3%, 9.3%, 11.8% respectively, while the overexpression rate of K-ras protein was 15.6%, 19.0%, 25.0%, 41.9%, 52.9% respectively. p53 protein expression was closely related with p53 mutation (P < 0.005, Pearson's R = 0.599 6). There was no relationship between the protein expression and gene mutation of K-ras (P > 0.500). CONCLUSIONS: p53 gene mutation and K-ras overexpression were early events in the carcinogenesis and development of rat lung carcinoma induced by MCA and DEN, while K-ras mutation does not play any important role. |
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Authors:
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Honggang Li; Mingqiu Liu; Luming Diao; Lunyin Yu; Honglei Chen; Fuchun Chen; Xuan Liu |
Publication Detail:
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Type: English Abstract; Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Zhonghua bing li xue za zhi Chinese journal of pathology Volume: 31 ISSN: 0529-5807 ISO Abbreviation: Zhonghua Bing Li Xue Za Zhi Publication Date: 2002 Aug |
Date Detail:
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Created Date: 2002-11-05 Completed Date: 2002-12-19 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 0005331 Medline TA: Zhonghua Bing Li Xue Za Zhi Country: China |
Other Details:
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Languages: chi Pagination: 331-6 Citation Subset: IM |
Affiliation:
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Department of Pathology, Medical College, Wuhan University, Wuhan 430071, China. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Genes, p53* Genes, ras* Humans Immunohistochemistry Lung Neoplasms / chemically induced, chemistry, genetics* Mice Mutation* Polymerase Chain Reaction Polymorphism, Single-Stranded Conformational Rats Tumor Suppressor Protein p53 / analysis ras Proteins / analysis |
| Chemical | |
Reg. No./Substance:
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0/Tumor Suppressor Protein p53; EC 3.6.5.2/ras Proteins |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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