| Microdialysis study of amino acid neurotransmitters in the spinal dorsal horn of patients undergoing microsurgical dorsal root entry zone lesioning. Technical note. | |
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MedLine Citation:
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PMID: 11147857 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The aim of this study was to develop, for the first time in the human spinal dorsal horn (DH), an in vivo method for the study of amino acids (AAs). A microdialysis technique was used to sample AAs in the extracellular fluid of the DH apex in eight patients in whom surgery in the dorsal root entry zone (DREZ) was performed. Before making microsurgical lesions, specific concentric-type microdialysis probes were implanted over a 60-minute period in the DREZ and directed to the DH apex (10 implantations). The AA concentrations in the dialysates were determined using high-performance liquid chromatography with fluorescence detection. The concentrations of excitatory AAs (glutamate and aspartate) and inhibitory AAs (gamma-aminobutyric acid and glycine) decreased and were stabilized by 45 minutes after probe implantation, whereas the levels of nonneurotransmitter AAs (alanine and threonine) were not stabilized at 60 minutes. The ability of the probe to track the changes of extracellular AAs was demonstrated. Neither intra- nor postoperative microdialysis-related complications were observed (with a follow up of 18 months). The present study demonstrates that microdialysis can be performed safely in the human DH during DREZ lesioning. Despite technical and analytical limitations related to the intraoperative conditions, this technique offers new possibilities for clinical research on neurotransmitters involved in some relevant pathological states, especially in chronic pain and spasticity. |
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Authors:
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P Mertens; C Ghaemmaghami; L Bert; A Perret-Liaudet; M Guenot; H Naous; L Laganier; R Later; M Sindou; B Renaud |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Journal of neurosurgery Volume: 94 ISSN: 0022-3085 ISO Abbreviation: J. Neurosurg. Publication Date: 2001 Jan |
Date Detail:
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Created Date: 2001-01-05 Completed Date: 2001-01-25 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 0253357 Medline TA: J Neurosurg Country: United States |
Other Details:
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Languages: eng Pagination: 165-73 Citation Subset: AIM; IM |
Affiliation:
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INSERM-U 512, Department of Neurosurgery, Federative Institute for Neuroscience, Claude Bernald University and Neurological Hospital, Hospices Civils de Lyon, France. patrick.mertens@chu-lyon.fr |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Adolescent Adult Amino Acids / metabolism* Female Humans Male Microdialysis Microsurgery* / methods Middle Aged Neurotransmitter Agents / metabolism* Osmolar Concentration Spinal Cord / metabolism* Spinal Nerve Roots / surgery* |
| Chemical | |
Reg. No./Substance:
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0/Amino Acids; 0/Neurotransmitter Agents |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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