| Microdialysis monitoring of 3,4-dihydroxyphenylalanine accumulation after decarboxylase inhibition: a means to estimate in vivo changes in tyrosine hydroxylase activity of the rat locus ceruleus. | |
| | |
MedLine Citation:
|
PMID: 8093483 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
An on-line microdialysis approach was developed to estimate changes in tyrosine hydroxylase activity in the locus ceruleus noradrenergic neurons of anesthetized rats by measuring the 3,4-dihydroxyphenylalanine (DOPA) accumulation in the extracellular fluid during perfusion of an aromatic amino acid decarboxylase inhibitor through a dialysis probe. The aromatic amino acid decarboxylase inhibitor used was difluoromethyl-DOPA, which was shown to be more stable than NSD 1015 or Ro 4-4602 in the perfusion fluid. A 1-h perfusion of a 10(-4) mol/L of difluoromethyl-DOPA solution induced a linear increase in DOPA concentration in the locus ceruleus dialysates that achieved a steady state within 1 h. The identity of DOPA accumulated in dialysates during aromatic amino acid decarboxylase inhibition was confirmed by the disappearance of the chromatographic peak when DOPA formation was blocked by the administration of alpha-methyl-p-tyrosine. Systemic administration of the alpha 2-antagonist piperoxane before difluoromethyl-DOPA perfusion markedly increased the DOPA concentration during both the accumulation and the steady-state periods, showing that the present technique is a suitable in vivo approach to monitor changes in tyrosine hydroxylase activity occurring in the locus ceruleus neurons. |
| | |
Authors:
|
F Robert; L Lambás-Señas; C Ortemann; J F Pujol; B Renaud |
Related Documents
:
|
2906363 - Csf gradients for amino acid neurotransmitters. 8412013 - Cerebrospinal fluid amino acids, purines and pyrimidines as a tool in the study of meta... 2347173 - Maleopimaric acid--a potent sensitizer in modified rosin. |
Publication Detail:
|
Type: Journal Article |
Journal Detail:
|
Title: Journal of neurochemistry Volume: 60 ISSN: 0022-3042 ISO Abbreviation: J. Neurochem. Publication Date: 1993 Feb |
Date Detail:
|
Created Date: 1993-02-09 Completed Date: 1993-02-09 Revised Date: 2003-11-14 |
Medline Journal Info:
|
Nlm Unique ID: 2985190R Medline TA: J Neurochem Country: UNITED STATES |
Other Details:
|
Languages: eng Pagination: 721-9 Citation Subset: IM |
Affiliation:
|
Université Claude Bernard et CNRS UMR 105, Laboratoire de Neuropharmacologie, Faculté de Pharmacie, Lyon, France. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
3,4-Dihydroxyphenylacetic Acid
/
metabolism Animals Aromatic-L-Amino-Acid Decarboxylases / antagonists & inhibitors* Benserazide / pharmacology* Chromatography, High Pressure Liquid Dialysis / methods Dihydroxyphenylalanine / analogs & derivatives*, metabolism*, pharmacology Hydrazines / pharmacology* Kinetics Locus Coeruleus / enzymology* Male Methyltyrosines / pharmacology* Microchemistry Rats Rats, Sprague-Dawley Time Factors Tyrosine 3-Monooxygenase / metabolism* alpha-Methyltyrosine |
| Chemical | |
Reg. No./Substance:
|
0/Hydrazines; 0/Methyltyrosines; 102-32-9/3,4-Dihydroxyphenylacetic Acid; 322-35-0/Benserazide; 63-84-3/Dihydroxyphenylalanine; 637-33-2/3-hydroxybenzylhydrazine; 658-48-0/alpha-Methyltyrosine; 69955-03-9/alpha-difluoromethyl-DOPA; EC 1.14.16.2/Tyrosine 3-Monooxygenase; EC 4.1.1.28/Aromatic-L-Amino-Acid Decarboxylases |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Phloretin as an antagonist of prostaglandin F2 alpha receptor in cultured rat astrocytes.
Next Document: Regulation of neuropeptide expression in cultured cerebral cortical neurons by brain-derived neurotr...