| Microdevice for analyzing the effect of electrochemotherapy on cancer cells. | |
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MedLine Citation:
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PMID: 19344145 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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A microfabricated cell-based testing device for electrochemotherapy (ECT) has been developed; it mimics a clinical electroporator with a circular needle array and maintains a similar electric field strength distribution. Until now, the performance between electroporators having two- and six-needle circular array electrodes, which are the general needle-type clinical electroporators for ECT, has not been evaluated systemically, although many studies have investigated the efficacy of ECT on cancer cells. In this study, the cell-based performance on the newly developed ECT testing device was analyzed in two- and six-electrode modes using propidium iodide and bleomycin, and the electroporation characteristics were characterized. With on-chip fluorescence-based assays, the proposed device offers time- and cost-effective experimental procedures compared with conventional assays, and the results can reflect the electric characteristics of clinical electroporators. |
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Authors:
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Youn-Suk Choi; Hong-Bae Kim; Seung-Hoon Kim; Jaekyu Choi; Je-Kyun Park |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Analytical chemistry Volume: 81 ISSN: 1520-6882 ISO Abbreviation: Anal. Chem. Publication Date: 2009 May |
Date Detail:
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Created Date: 2009-04-30 Completed Date: 2009-06-25 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 0370536 Medline TA: Anal Chem Country: United States |
Other Details:
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Languages: eng Pagination: 3517-22 Citation Subset: IM |
Affiliation:
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Department of Bio and Brain Engineering, College of Life Science and Bioengineering, KAIST, 335 Gwahangno, Yuseong-gu, Daejeon 305-701, Republic of Korea. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Bleomycin
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metabolism,
pharmacology,
therapeutic use Breast Neoplasms / drug therapy*, pathology* Carcinoma, Ductal / drug therapy*, pathology* Cell Line, Tumor Cell Proliferation / drug effects Electrochemotherapy* Electrodes Fluorescence Humans Microtechnology / instrumentation* Propidium / metabolism, therapeutic use |
| Chemical | |
Reg. No./Substance:
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11056-06-7/Bleomycin; 36015-30-2/Propidium |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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