Document Detail


Microdevice for analyzing the effect of electrochemotherapy on cancer cells.
MedLine Citation:
PMID:  19344145     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
A microfabricated cell-based testing device for electrochemotherapy (ECT) has been developed; it mimics a clinical electroporator with a circular needle array and maintains a similar electric field strength distribution. Until now, the performance between electroporators having two- and six-needle circular array electrodes, which are the general needle-type clinical electroporators for ECT, has not been evaluated systemically, although many studies have investigated the efficacy of ECT on cancer cells. In this study, the cell-based performance on the newly developed ECT testing device was analyzed in two- and six-electrode modes using propidium iodide and bleomycin, and the electroporation characteristics were characterized. With on-chip fluorescence-based assays, the proposed device offers time- and cost-effective experimental procedures compared with conventional assays, and the results can reflect the electric characteristics of clinical electroporators.
Authors:
Youn-Suk Choi; Hong-Bae Kim; Seung-Hoon Kim; Jaekyu Choi; Je-Kyun Park
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Analytical chemistry     Volume:  81     ISSN:  1520-6882     ISO Abbreviation:  Anal. Chem.     Publication Date:  2009 May 
Date Detail:
Created Date:  2009-04-30     Completed Date:  2009-06-25     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0370536     Medline TA:  Anal Chem     Country:  United States    
Other Details:
Languages:  eng     Pagination:  3517-22     Citation Subset:  IM    
Affiliation:
Department of Bio and Brain Engineering, College of Life Science and Bioengineering, KAIST, 335 Gwahangno, Yuseong-gu, Daejeon 305-701, Republic of Korea.
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MeSH Terms
Descriptor/Qualifier:
Bleomycin / metabolism,  pharmacology,  therapeutic use
Breast Neoplasms / drug therapy*,  pathology*
Carcinoma, Ductal / drug therapy*,  pathology*
Cell Line, Tumor
Cell Proliferation / drug effects
Electrochemotherapy*
Electrodes
Fluorescence
Humans
Microtechnology / instrumentation*
Propidium / metabolism,  therapeutic use
Chemical
Reg. No./Substance:
11056-06-7/Bleomycin; 36015-30-2/Propidium

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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