| Microcystin-induced activation of prostaglandin synthesis and phospholipid metabolism in rat hepatocytes. | |
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MedLine Citation:
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PMID: 20732035 Owner: NLM Status: In-Data-Review |
Abstract/OtherAbstract:
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The effects of microcystin-LR, a trichothecene, T-2 and saxitoxin on membrane lipid mediators of inflammatory processes were evaluated in cultured rat hepatocytes using [(14)C]arachidonic acid. Microcystin-LR significantly stimulated the release of prostacyclin, measured as 6-keto PGF(1)alpha, by 38%, and thromboxane B(2) by 50%, in a concentration-dependent manner. The trichothecene toxin T-2 enhanced the release of prostaglandin F(2)alpha by 24% and arachidonic acid by 29%; while saxitoxin did not affect the release prostaglandins or arachidonic acid. Incorporation of arachidonic acid into the lipid pool was reduced by 47% by 1 mum microcystin-LR. Changes in the distribution of radioactivity derived from [(14)C]arachidonic acid within phospholipid classes indicated that prostaglandin formation induced by microcystin-LR was also due to the release of arachidonic acid from the phosphatidylinositol pool. No statistically significant effect of toxin was observed on the contribution of [(14)C]arachidonic acid release by other classes of phospholipids or neutral lipids. These effects may be important in the mechanism of microcystin-LR-induced toxicity in the liver. |
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Authors:
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S M Naseem; K A Mereish; R Solow; H B Hines |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Toxicology in vitro : an international journal published in association with BIBRA Volume: 5 ISSN: 0887-2333 ISO Abbreviation: Toxicol In Vitro Publication Date: 1991 |
Date Detail:
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Created Date: 2010-08-24 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 8712158 Medline TA: Toxicol In Vitro Country: England |
Other Details:
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Languages: eng Pagination: 341-5 Citation Subset: - |
Affiliation:
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Pathophysiology Division, US Army Medical Research Institute of Infectious Diseases, Fort Detrick, Frederick, MD 21702-5011, USA. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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