Document Detail

Microcomputer-aided reconstruction: a system designed for the study of 3-D microstructure in histology and histopathology.
MedLine Citation:
PMID:  3298657     Owner:  NLM     Status:  MEDLINE    
We have designed a microcomputer system to reproduce 3-D tissue structures graphically from serial microscopic sections. The system, based on a Hewlett-Packard model 310 desktop computer, comprises a floppy disc drive, a hard-disc that extends the available user memory by adding 40 Mbytes, a colour memory-mapped graphics display, a graphics tablet and a graphics printer. A set of serial 2-D images, manually extracted from serial sections using a microprojector, are digitized on the tablet by tracing the contours of the structure of interest; up to ten different structures can be inputted and reproduced using a 'hidden line' effect. The software, written mainly in HP-BASIC 4.0, produces 3-D pictures of a tissue structure either as a 'stack of slices' with or without being 'tiled' with triangular patches, or in 'wire framing'. Any angle of rotation around the x, y and z axes is assigned for the image to be reconstructed, allowing the operator to obtain the best perspective. The system also discloses the internal connectivity of an object by reducing the structure to a network diagram; this is especially useful in analysing the topological properties of tissue structure. It is shown how, in some examples, the system contributes to a better understanding of tissue microstructures and their morbid changes and how, as an effective tool in morphology, it will aid future studies of histology and histopathology.
H Yaegashi; T Takahashi; M Kawasaki
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Journal of microscopy     Volume:  146     ISSN:  0022-2720     ISO Abbreviation:  J Microsc     Publication Date:  1987 Apr 
Date Detail:
Created Date:  1987-08-07     Completed Date:  1987-08-07     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0204522     Medline TA:  J Microsc     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  55-65     Citation Subset:  IM    
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MeSH Terms
Adenocarcinoma / pathology
Bronchitis / pathology
Histological Techniques*
Liver Cirrhosis / pathology
Lung / pathology
Stomach Neoplasms / pathology

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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