Document Detail

Microcin E492, a channel-forming bacteriocin from Klebsiella pneumoniae, induces apoptosis in some human cell lines.
MedLine Citation:
PMID:  11880624     Owner:  NLM     Status:  MEDLINE    
The cytotoxic effect of microcin E492, a low-molecular-mass channel-forming bacteriocin (7,887 Da) produced by a strain of Klebsiella pneumoniae, was characterized in HeLa cells. At low (5 microg/ml) and intermediate (10 microg/ml) concentrations, microcin E492 induced biochemical and morphological changes typical of apoptosis, such as cell shrinkage, DNA fragmentation, extracellular exposure of phosphatidylserine, caspase activation, and loss of mitochondrial membrane potential. Treatment with zVAD-fmk, a general caspase inhibitor, completely blocked the cytotoxic effect of this bacteriocin. At higher microcin concentrations (>20 microg/ml) a necrotic phenotype was observed. Induction of apoptosis by microcin E492 was associated with the release of calcium from intracellular stores, probably after microcin-triggered ion channel formation. Microcin E492 also presented a cytotoxic effect on Jurkat and RJ2.25 cells, but had no effect on KG-1 cells nor on a primary culture of human tonsil endothelial cells, suggesting that there is a specific interaction of the bacteriocin with components of the target cell surface. This report describes a bacteriocin that has the capacity to induce apoptosis in human cell lines.
Claudio Hetz; María Rosa Bono; Luis Felipe Barros; Rosalba Lagos
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Proceedings of the National Academy of Sciences of the United States of America     Volume:  99     ISSN:  0027-8424     ISO Abbreviation:  Proc. Natl. Acad. Sci. U.S.A.     Publication Date:  2002 Mar 
Date Detail:
Created Date:  2002-03-07     Completed Date:  2002-04-26     Revised Date:  2009-11-18    
Medline Journal Info:
Nlm Unique ID:  7505876     Medline TA:  Proc Natl Acad Sci U S A     Country:  United States    
Other Details:
Languages:  eng     Pagination:  2696-701     Citation Subset:  IM    
Departamento de Biología, Facultad de Ciencias, Universidad de Chile, Casilla 653, Santiago, Chile.
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MeSH Terms
Anti-Bacterial Agents / pharmacology*
Bacteriocins / pharmacology*
Calcium / metabolism
Caspases / metabolism
Cell Line
Cytochrome c Group / metabolism
Cytotoxins / pharmacology*
Enzyme Activation
Hela Cells
Intracellular Membranes / drug effects
Jurkat Cells
Klebsiella pneumoniae*
Membrane Potentials / drug effects
Mitochondria / drug effects
Tumor Cells, Cultured
Reg. No./Substance:
0/Anti-Bacterial Agents; 0/Bacteriocins; 0/Cytochrome c Group; 0/Cytotoxins; 1403-96-9/microcin; 7440-70-2/Calcium; EC 3.4.22.-/Caspases

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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