Document Detail


Microchip-based enumeration of human white blood cells.
MedLine Citation:
PMID:  18365704     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The advent of flow cytometry has considerably changed the ways in which medical testing is conducted. However, the cost of flow cytometers, their large size, and their maintenance needs make them scarce in resource-poor settings and available almost only in clinical pathology laboratories in developed countries. Because cell enumeration is a basic and crucial support of diagnosis, prognosis, and treatment, an alternative cell-counting method that would potentially be cost-effective, portable, and suitable for use in resource-poor settings is warranted. We describe here a protocol for conducting cell-counting experiments in a simple microfluidic structure. This protocol describes how to build a simple microfluidic cell and perform a total white blood cell (WBC) count through capture and immunolabeling of the WBCs with an anti-CD45 antibody.
Authors:
Pierre N Floriano; Shelley Acosta; Nicolaos Christodoulides; Shannon Weigum; John T McDevitt
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Methods in molecular biology (Clifton, N.J.)     Volume:  385     ISSN:  1064-3745     ISO Abbreviation:  Methods Mol. Biol.     Publication Date:  2007  
Date Detail:
Created Date:  2008-03-27     Completed Date:  2008-07-02     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9214969     Medline TA:  Methods Mol Biol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  53-64     Citation Subset:  IM    
Affiliation:
Department of Chemistry and Biochemistry, University of Texas at Austin, USA.
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MeSH Terms
Descriptor/Qualifier:
Antigens, CD45 / immunology
Flow Cytometry
Humans
Leukocyte Count / instrumentation,  methods*
Leukocytes / cytology*
Microfluidics / instrumentation,  methods
Grant Support
ID/Acronym/Agency:
U01-DE0015017-03/DE/NIDCR NIH HHS
Chemical
Reg. No./Substance:
EC 3.1.3.48/Antigens, CD45

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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