Document Detail


Microchamber arrays for the identification of individual cells exposed to an X-ray microbeam.
MedLine Citation:
PMID:  18584192     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
To identify individual cells exposed to a X-ray microbeam in a cell population, we developed a biocompatible microchamber-array chip using UV lithography of photopolymer SU-8. The center-to-center distance between microchambers is 50 mum including a wall of 15 mum height. Using the microchamber-array chip, we performed tracking of individual exposed cells. Sample cells loaded in a microchamber array were selectively irradiated with the X-ray microbeam under microscopic observation. All the irradiated cells were indexed by the array arrangement of the microchambers. For about 24 h of post-irradiation incubation, the irradiated cells were identified successfully by time-lapse observation. In addition, the induction of radiation effects was observed in identified cells using immunofluorescence.
Authors:
Takahiro Kuchimaru; Fuminobu Sato; Yusuke Aoi; Tomohisa Fujita; Toshiji Ikeda; Kikuo Shimizu; Yushi Kato; Toshiyuki Iida
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2008-06-27
Journal Detail:
Title:  Radiation and environmental biophysics     Volume:  47     ISSN:  0301-634X     ISO Abbreviation:  Radiat Environ Biophys     Publication Date:  2008 Nov 
Date Detail:
Created Date:  2008-10-13     Completed Date:  2009-02-12     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0415677     Medline TA:  Radiat Environ Biophys     Country:  Germany    
Other Details:
Languages:  eng     Pagination:  535-40     Citation Subset:  IM    
Affiliation:
Division of Electric, Electronic and Information Engineering, Graduate School of Engineering, Osaka University, Suita, Osaka 567-0871, Japan. kuchimaru@nf.eie.eng.osaka-u.ac.jp
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MeSH Terms
Descriptor/Qualifier:
Animals
Cell Culture Techniques / instrumentation*
Cell Separation / instrumentation*
Equipment Design
Equipment Failure Analysis
Microarray Analysis / instrumentation*
Neurons / physiology*,  radiation effects*
PC12 Cells
Radiation Dosage
Radiometry / instrumentation*
Rats
X-Rays

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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