Document Detail


Microcarrier enhanced survival of human and rat fetal ventral mesencephalon cells implanted in the rat striatum.
MedLine Citation:
PMID:  9440867     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The transplantation of tissue containing dopamine-producing cells into the mammalian central nervous system is an emerging treatment for Parkinson's disease, despite relatively poor survival of implanted tissue. Recent evidence has suggested that Cytodex microcarriers enhance the survival of dopaminergic rat chromaffin cells transplanted into the rat striatum in the absence of immunosuppression. The current study was undertaken to evaluate the survival of rat and human fetal ventral mesencephalic neurons (VM) implanted alone or after attachment to microcarriers in the striatum of rats without immunosuppression. Rat fetal VM neurons demonstrated enhanced survival in the rat striatum when transplanted on microcarriers, compared to their transplantation alone during the 3-mo period examined in the present study. Transplants of human fetal VM neurons on microcarriers also survived remarkably well in the rat striatum without systemic immunosuppression. In contrast, human fetal VM cells transplanted alone into the rat striatum did not survive without systemic immunosuppression. There was no evidence of TH fiber sprouting in the vicinity of any transplant site. These data indicated that Cytodex microcarriers provide enhanced survival of both rat allograft and human xenograft fetal mesencephalic cells in the rat striatum without the necessity of systemic immunosuppression, perhaps by inducing a unique neuron-glia environment.
Authors:
S Saporta; C Borlongan; J Moore; E Mejia-Millan; S L Jones; P Bonness; T S Randall; R C Allen; T B Freeman; P R Sanberg
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Cell transplantation     Volume:  6     ISSN:  0963-6897     ISO Abbreviation:  Cell Transplant     Publication Date:    1997 Nov-Dec
Date Detail:
Created Date:  1998-02-12     Completed Date:  1998-02-12     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  9208854     Medline TA:  Cell Transplant     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  579-84     Citation Subset:  IM    
Affiliation:
Department of Anatomy, University of South Florida College of Medicine, Tampa 33612-4799, USA.
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MeSH Terms
Descriptor/Qualifier:
Animals
Brain Tissue Transplantation / methods*
Cell Transplantation / methods*
Corpus Striatum
Dextrans
Fetal Tissue Transplantation / methods*
Graft Survival*
Humans
Male
Mesencephalon / cytology*,  embryology
Microspheres
Neurons / transplantation*
Oxidopamine
Parkinson Disease, Secondary / chemically induced,  surgery
Rats
Rats, Sprague-Dawley
Transplantation Conditioning
Transplantation, Heterologous
Transplantation, Homologous
Chemical
Reg. No./Substance:
1199-18-4/Oxidopamine; 79920-52-8/Cytodex; 9004-54-0/Dextrans

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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