Document Detail


Microcapillary specifically designed for pressure microinjections of very low volumes.
MedLine Citation:
PMID:  20576508     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Tracer micronjections are very widely used in brain mapping research. While administration of small quantities/volumes of tracers can easily be achieved through iontophoresis, in the case of pressure injections the volume of the substances injected are much more difficult to control. Instead of using different variables like hydrodynamic conductance, pressure pulses, pressure-temperature-dependent protocols to quantify very small volumes in the nanoliter range out of a higher volume, within the confines of the present study a novel microcapillary design is presented. This microcapillary contains exactly the volume of tracer one intends to inject, therefore the danger of flooding large brain areas or the risk of tracer leakage to neighbouring nuclei are completely eliminated, and in the same time this design assures that very small and circumscribed areas can be labeled and their connections mapped, thus making the experiments more specific. In combination with high precision stereotaxic measurements these small volumes of tracers can yield well targeted and very discrete injection sites that make possible the mapping of individual nuclear subdivisions or delicate nuclei in the brain.
Authors:
Zsolt István Kovács; Miklós Palkovits
Related Documents :
3703208 - Acute middle cerebral artery occlusion: experience with volume expansion therapy.
16311438 - The arterial system pressure-volume loop.
3794518 - Effect of hyponatremia on response to volume depletion in uremic dogs.
12911228 - Measurement of enthalpy and volume changes in photoinitiated reactions on the ms timesc...
21743258 - Impact of a mass media campaign to increase public awareness of hypertension.
10023748 - Analysis of right ventricular areas to assess the severity of ascites syndrome in broil...
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2010-06-01
Journal Detail:
Title:  Journal of neuroscience methods     Volume:  190     ISSN:  1872-678X     ISO Abbreviation:  J. Neurosci. Methods     Publication Date:  2010 Jul 
Date Detail:
Created Date:  2010-07-05     Completed Date:  2010-10-22     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  7905558     Medline TA:  J Neurosci Methods     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  229-34     Citation Subset:  IM    
Copyright Information:
Copyright 2010 Elsevier B.V. All rights reserved.
Affiliation:
Neuromorphological and Neuroendocrine Research Laboratory, Department of Anatomy, Semmelweis University and the Hungarian Academy of Sciences, Budapest, Hungary. kzsoltdr@yahoo.com
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:
Algorithms
Animals
Brain / cytology,  virology
Cholera Toxin / administration & dosage
Equipment Design
Herpesvirus 1, Suid
Immunohistochemistry
Male
Microfluidics / instrumentation*,  methods
Microinjections / instrumentation*,  methods
Neuronal Tract-Tracers / administration & dosage
Phytohemagglutinins / administration & dosage
Pressure
Rats
Rats, Sprague-Dawley
Water
Chemical
Reg. No./Substance:
0/Neuronal Tract-Tracers; 0/Phytohemagglutinins; 0/leukoagglutinins, plants; 7732-18-5/Water; 9012-63-9/Cholera Toxin

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Bilateral cochlear implantation in the ferret: a novel animal model for behavioral studies.
Next Document:  Fish consumption, mercury exposure, and their associations with scholastic achievement in the Seyche...