Document Detail


Microbore high-performance liquid chromatographic method for the measurement of dopamine and its metabolites: recommendations for optimal sample collection and storage.
MedLine Citation:
PMID:  7532076     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
A microbore high-performance liquid chromatographic method with electrochemical detection was developed for the measurement of small quantities of dopamine and its metabolites, 3,4-dihydroxyphenylacetic acid and homovanillic acid, in microdialysis samples from rat striatum. The limit of detection of dopamine was in the low nmol/l range (10 fmol/40 microliters dialysate). Standard mixtures of dopamine and its metabolites were stored in tubes made of polypropylene, soda-lime glass or borosilicate glass. Dopamine and homovanillic acid were stable in all three types of container for 24 h providing they were kept at an acidic pH. However, 3,4-dihydroxyphenylacetic acid was only stable in tubes made of polypropylene or borosilicate glass. Basal levels of dopamine in the dialysate obtained from rats which had been chronically implanted with a microdialysis probe in the anterior striatum were stable for one to three days following probe implantation. However, the levels of 3,4-dihydroxyphenylacetic acid and homovanillic acid in the dialysate decreased rapidly with time after the first day. This indicates that the synthesizing capacity of the neurons is compromised by the microdialysis probe after day one, and that microdialysis experiments in freely moving animals should be confined to the day immediately following probe implantation.
Authors:
A J Carter
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Journal of chromatography. B, Biomedical applications     Volume:  660     ISSN:  1572-6495     ISO Abbreviation:  J. Chromatogr. B, Biomed. Appl.     Publication Date:  1994 Oct 
Date Detail:
Created Date:  1995-03-22     Completed Date:  1995-03-22     Revised Date:  2007-10-16    
Medline Journal Info:
Nlm Unique ID:  9421796     Medline TA:  J Chromatogr B Biomed Appl     Country:  NETHERLANDS    
Other Details:
Languages:  eng     Pagination:  158-63     Citation Subset:  IM    
Affiliation:
Department of Pharmacology, Boehringer Ingelheim KG, Germany.
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MeSH Terms
Descriptor/Qualifier:
3,4-Dihydroxyphenylacetic Acid / analysis
Animals
Chromatography, High Pressure Liquid
Dopamine / analysis*
Homovanillic Acid / analysis
Hydroxyindoleacetic Acid / analysis
Indicators and Reagents
Microdialysis
Neostriatum / chemistry
Rats
Specimen Handling
Chemical
Reg. No./Substance:
0/Indicators and Reagents; 102-32-9/3,4-Dihydroxyphenylacetic Acid; 306-08-1/Homovanillic Acid; 54-16-0/Hydroxyindoleacetic Acid

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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