Document Detail


Microbore flow-rates and protein chromatography.
MedLine Citation:
PMID:  3558596     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Reversed-phase chromatography of proteins on microbore columns can achieve sensitivities that exceed those for standard-bore columns by a factor of 10-20, when operated at the same linear velocities. These gains in sensitivity are accompanied by proportional reductions in peak volume. Sensitivities on standard- (4.6 mm I.D.) and narrow-bore (2.1 mm I.D.) columns have been further improved by reducing the flow-rates to those typical for microbore (1 mm I.D.) columns. We have investigated the role of flow-rate in determining peak volumes for a constant time gradient and found that flow-rate affects peak volume to a much greater extent than column diameter. Column length was not found to have a significant effect on either peak volume or sensitivity. We have found that a four-fold reduction in flow-rate results in at least a two-fold reduction in peak volume over the flow-rate range from 25 to 400 microliters/min. Recovery of proteins in smaller volumes should prove beneficial to subsequent protein characterization methodologies.
Authors:
T D Schlabach; K J Wilson
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Journal of chromatography     Volume:  385     ISSN:  -     ISO Abbreviation:  J. Chromatogr.     Publication Date:  1987 Jan 
Date Detail:
Created Date:  1987-05-04     Completed Date:  1987-05-04     Revised Date:  2013-12-13    
Medline Journal Info:
Nlm Unique ID:  0427043     Medline TA:  J Chromatogr     Country:  NETHERLANDS    
Other Details:
Languages:  eng     Pagination:  65-74     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Chromatography, High Pressure Liquid
Humans
Hydrolysis
Ovalbumin / isolation & purification
Peptides / isolation & purification
Proteins / analysis*
Trypsin
Chemical
Reg. No./Substance:
0/Peptides; 0/Proteins; 9006-59-1/Ovalbumin; EC 3.4.21.4/Trypsin

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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