Document Detail


Microbiome diversity in the bronchial tracts of patients with chronic obstructive pulmonary disease.
MedLine Citation:
PMID:  22915614     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Culture of bacteria from bronchial secretions in respiratory patients has low sensitivity and does not allow for complete assessment of microbial diversity across different bronchial compartments. In addition, a significant number of clinical studies are based on sputum samples, and it is not known to what extent they describe the real diversity of the mucosa. In order to identify previously unrecognized lower airway bacteria and to investigate the complexity and distribution of microbiota in patients with chronic obstructive pulmonary disease (COPD), we performed PCR amplification and pyrosequencing of the 16S rRNA gene in patients not showing signs or symptoms of infection. Four types of respiratory samples (sputum, bronchial aspirate, bronchoalveolar lavage, and bronchial mucosa) were taken from each individual, obtaining on average >1,000 16S rRNA sequences per sample. The total number of genera per patient was >100, showing a high diversity, with Streptococcus, Prevotella, Moraxella, Haemophilus, Acinetobacter, Fusobacterium, and Neisseria being the most commonly identified. Sputum samples showed significantly lower diversity than the other three sample types. Lower-bronchial-tree samples, i.e., bronchoalveolar lavage and bronchial mucosa, showed a very similar bacterial compositions in contrast to sputum and bronchial aspirate samples. Thus, sputum and bronchial aspirate samples are upper bronchial tree samples that are not representative of the lower bronchial mucosa flora, and bronchoalveolar lavage samples showed the results closest to those for the bronchial mucosa. Our data confirm that the bronchial tree is not sterile in COPD patients and support the existence a different microbiota in the upper and lower compartments.
Authors:
Raúl Cabrera-Rubio; Marian Garcia-Núñez; Laia Setó; Josep M Antó; Andrés Moya; Eduard Monsó; Alex Mira
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2012-08-22
Journal Detail:
Title:  Journal of clinical microbiology     Volume:  50     ISSN:  1098-660X     ISO Abbreviation:  J. Clin. Microbiol.     Publication Date:  2012 Nov 
Date Detail:
Created Date:  2012-10-15     Completed Date:  2013-03-14     Revised Date:  2013-07-12    
Medline Journal Info:
Nlm Unique ID:  7505564     Medline TA:  J Clin Microbiol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  3562-8     Citation Subset:  IM    
Affiliation:
Department of Genomics and Health, Center for Public Health Research, Valencia, Spain.
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MeSH Terms
Descriptor/Qualifier:
Aged
Bacteria / classification*,  genetics*
Biota*
Bronchi / microbiology*
DNA, Bacterial / chemistry,  genetics
DNA, Ribosomal / chemistry,  genetics
Female
Humans
Male
Metagenome*
Molecular Sequence Data
Pulmonary Disease, Chronic Obstructive / microbiology*
RNA, Ribosomal, 16S / genetics
Sequence Analysis, DNA
Sputum / microbiology
Chemical
Reg. No./Substance:
0/DNA, Bacterial; 0/DNA, Ribosomal; 0/RNA, Ribosomal, 16S
Comments/Corrections

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