Document Detail

Microbioluminometry assay: determination of erythromycin activity in plasma or serum.
MedLine Citation:
PMID:  2614699     Owner:  NLM     Status:  MEDLINE    
A microbioluminometry assay (MBA) was developed for the quantitative analysis of erythromycin activity in human plasma or serum. The MBA method is adapted from turbidimetric methods and utilizes an enzyme-catalyzed bioluminescence reaction to quantitate the growth of Staphylococcus aureus in liquid culture medium. Statistical analysis of data obtained in method validation studies and in more than 500 assays of standard curve and control samples demonstrates consistent reproducibility and accuracy within theoretical limits. The MBA was shown to be more sensitive than agar diffusion assays with a lower limit of sensitivity less than 20.0 ng/mL and coefficients of variation less than 10%. Cumulative results of 178 assays of spiked control plasma samples in the range of 0.14-2.18 micrograms/mL show 11.2% of individual determinations are greater than +/- 15% the expected value, and 5.6% of individual determinations are greater than +/- 20% the expected value. Bioavailability profiles obtained with MBA are consistent with reported data for erythromycin. Values for 206 subject samples analyzed by MBA and agar diffusion assays showed a high degree of correlation (r = 0.9525) between the two methods. The MBA technique provided high sample throughput because of the use of microtiter plate technologies; it is also economical since it requires less sample and reagents.
P E Andreotti; I S Morse; D M Hartmann
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Journal of pharmaceutical sciences     Volume:  78     ISSN:  0022-3549     ISO Abbreviation:  J Pharm Sci     Publication Date:  1989 Dec 
Date Detail:
Created Date:  1990-02-28     Completed Date:  1990-02-28     Revised Date:  2004-11-17    
Medline Journal Info:
Nlm Unique ID:  2985195R     Medline TA:  J Pharm Sci     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  979-85     Citation Subset:  IM    
South Florida Bioavailability Clinic, Research and Development, Fort Lauderdale 33334.
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MeSH Terms
Biological Availability
Erythromycin / blood*,  pharmacokinetics,  pharmacology
Luminescent Measurements
Quality Control
Regression Analysis
Staphylococcus aureus / analysis,  drug effects
Reg. No./Substance:

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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