Document Detail


Microbiology Diagnosis of chlamydia trachomatis infection.
MedLine Citation:
PMID:  6177643     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The etiological diagnosis of what is today known as infection by Chlamydia trachomatis was first made possible in 1907 when Halberstaedter and von Prowazek identified inclusions in conjunctival scrapings by means of Giemsa staining. C. trachomatis was originally classified as a virus, and the culture systems used were those for viruses. Macchiavello was the first to describe the isolation of C. trachomatis in embryonated hens' eggs (1944), but the first isolation is usually credited to T'ang and co-workers (1957), also using eggs. A major step in the understanding of chlamydial infections was made in 1965 when Gordon and Quan published a paper on the use of irradiated McCoy cells for isolation purposes. This technique made it possible to perform cultures from genital specimens with simplicity in comparison to isolation from eggs. Various culture techniques have been developed parallel to the expanding knowledge of the basic biology of the genus Chlamydia. McCoy cells (mouse fibroblasts), HeLa 229 (derived from human cervical carcinoma cells) and BHK-21 cells (baby hamster kidney cells) are the cell types regularly used for the culture of C. trachomatis. The principles underlying the various culture techniques are discussed. A description of the original irradiated McCoy cell system and the simplified, sensitive technique using cycloheximide-treated McCoy cells are given in this paper.
Authors:
K T Ripa
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Publication Detail:
Type:  Journal Article; Review    
Journal Detail:
Title:  Infection     Volume:  10 Suppl 1     ISSN:  0300-8126     ISO Abbreviation:  Infection     Publication Date:  1982  
Date Detail:
Created Date:  1982-08-07     Completed Date:  1982-08-07     Revised Date:  2005-11-17    
Medline Journal Info:
Nlm Unique ID:  0365307     Medline TA:  Infection     Country:  GERMANY, WEST    
Other Details:
Languages:  eng     Pagination:  S19-24     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Animals
Cell Division
Cells, Cultured
Centrifugation
Chlamydia trachomatis / isolation & purification*,  physiology
Cricetinae
Cycloheximide / pharmacology
Female
Humans
Lymphogranuloma Venereum / diagnosis*,  microbiology
Phagocytosis
Polyamines*
Polymers / pharmacology
Staining and Labeling
Trachoma / diagnosis*,  microbiology
Chemical
Reg. No./Substance:
0/Polyamines; 0/Polymers; 0/polycations; 66-81-9/Cycloheximide

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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