Document Detail


Microbicidal and cytotoxic effects of functional water in vitro.
MedLine Citation:
PMID:  20806093     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
OBJECTIVE: Several kinds of functional water are used in the fields of food hygiene and medicine. The purpose of this study was to evaluate both the disinfection and cytotoxic effects of functional water in comparison with commonly used root canal irrigants such as sodium hypochlorite solution and hydrogen peroxide solution.
METHOD AND MATERIALS: Three kinds of functional water were examined: alkaline electrolysis water (AEW), strong acid electrolyzed water (SAEW), and hypochlorous acid water (HAW). The disinfection effect was studied using Enterococcus faecalis and Candida albicans with or without organic substance. Each kind of functional water was applied to samples, and the colony formation was evaluated. The cytotoxic effect was evaluated by mitogenic assay (MTT) and alkaline phosphatase (ALPase) activity in pulp cells.
RESULTS: SAEW and HAW showed microbicidal effects in the presence of organic substance, with an effect almost similar to sodium hypochlorite solution. AEW did not show any microbicidal effect. SAEW, AEW, and HAW at 10- and 1,000-times dilution did not inhibit the MTT assay and ALPase activity. The cytotoxicity of SAEW and HAW against pulp cells was mild compared to that of sodium hypochlorite solution.
CONCLUSION: Functional water like SAEW and HAW have a good microbicidal effect under existing organic substance and are also mild to pulp cells.
Authors:
Kazuhiro Gomi; Tomohiko Makino; Shinichi Suzuki; Masako Hasegawa; Nobuko Maeda; Takashi Arai
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Publication Detail:
Type:  Comparative Study; Journal Article    
Journal Detail:
Title:  Quintessence international (Berlin, Germany : 1985)     Volume:  41     ISSN:  1936-7163     ISO Abbreviation:  Quintessence Int     Publication Date:  2010 Oct 
Date Detail:
Created Date:  2010-08-31     Completed Date:  2010-12-23     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0342677     Medline TA:  Quintessence Int     Country:  Germany    
Other Details:
Languages:  eng     Pagination:  e166-72     Citation Subset:  D    
Affiliation:
Department of Endodontics and Periodontics, Tsurumi University, School of Dental Medicine, Yokohama, Japan. gomi-k@tsurumi-u.ac.jp
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MeSH Terms
Descriptor/Qualifier:
Alkalies
Alkaline Phosphatase / analysis
Candida albicans / drug effects
Cell Culture Techniques
Cell Proliferation / drug effects
Cell Survival / drug effects
Colony Count, Microbial
Coloring Agents / diagnostic use
Dental Pulp / cytology,  drug effects*
Disinfectants / pharmacology*,  toxicity
Enterococcus faecalis / drug effects
Humans
Hydrogen Peroxide / pharmacology,  toxicity
Hypochlorous Acid / pharmacology,  toxicity
Root Canal Irrigants / pharmacology*,  toxicity
Sodium Hypochlorite / pharmacology,  toxicity
Tetrazolium Salts / diagnostic use
Thiazoles / diagnostic use
Water
Chemical
Reg. No./Substance:
0/Alkalies; 0/Coloring Agents; 0/Disinfectants; 0/Root Canal Irrigants; 0/Tetrazolium Salts; 0/Thiazoles; 0/superoxidized water; 298-93-1/thiazolyl blue; 7681-52-9/Sodium Hypochlorite; 7722-84-1/Hydrogen Peroxide; 7732-18-5/Water; 7790-92-3/Hypochlorous Acid; EC 3.1.3.1/Alkaline Phosphatase

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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