Document Detail


Microbial utilization and selectivity of pectin fractions with various structures.
MedLine Citation:
PMID:  21724897     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
To evaluate the fermentation properties of oligosaccharides derived from pectins and their parent polysaccharides, a 5-ml-working-volume, pH- and temperature-controlled fermentor was tested. Six pectic oligosaccharides representing specific substructures found within pectins were prepared. These consisted of oligogalacturonides (average degrees of polymerization [DP] of 5 and 9), methylated oligogalacturonides (average DP of 5), oligorhamnogalacturonides (average DP of 10 as a disaccharide unit of galacturonic acid and rhamnose), oligogalactosides (average DP of 5), and oligoarabinosides (average DP of 6). The influence of these carbohydrates on the human fecal microbiota was evaluated. Use of neutral sugar fractions resulted in an increase in Bifidobacterium populations and gave higher organic acid yields. The Bacteroides-Prevotella group significantly increased on all oligosaccharides except oligogalacturonides with an average DP of 5. The most selective substrates for bifidobacteria were arabinan, galactan, oligoarabinosides, and oligogalactosides.
Authors:
Chatchaya Onumpai; Sofia Kolida; Estelle Bonnin; Robert A Rastall
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Validation Studies     Date:  2011-07-01
Journal Detail:
Title:  Applied and environmental microbiology     Volume:  77     ISSN:  1098-5336     ISO Abbreviation:  Appl. Environ. Microbiol.     Publication Date:  2011 Aug 
Date Detail:
Created Date:  2011-08-05     Completed Date:  2011-12-15     Revised Date:  2012-02-01    
Medline Journal Info:
Nlm Unique ID:  7605801     Medline TA:  Appl Environ Microbiol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  5747-54     Citation Subset:  IM    
Affiliation:
Department of Food and Nutritional Sciences, University of Reading, Whiteknights, Reading, United Kingdom.
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MeSH Terms
Descriptor/Qualifier:
Analysis of Variance
Arabidopsis / chemistry
Bacterial Load / methods
Bacteroides / metabolism
Bifidobacterium / metabolism*
Bioreactors / microbiology*,  standards
Chemical Fractionation / methods
Fatty Acids, Volatile / metabolism
Feces / microbiology*
Fermentation*
Hexuronic Acids / metabolism
Humans
In Situ Hybridization, Fluorescence
Oligosaccharides / metabolism
Pectins / metabolism*
Rhamnose / metabolism
Seeds / chemistry
Chemical
Reg. No./Substance:
0/Fatty Acids, Volatile; 0/Hexuronic Acids; 0/Oligosaccharides; 0/Pectins; 10485-94-6/Rhamnose; 14982-50-4/galacturonic acid

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