| Microbial and "de novo" transformation of dicarboxylic acids by three airborne fungi. | |
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MedLine Citation:
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PMID: 18037475 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Micro-organisms and organic compounds of biogenic or anthropogenic origins are important constituents of atmospheric aerosols, which are involved in atmospheric processes and climate change. In order to investigate the role of fungi and their metabolisation activity, we collected airborne fungi using a biosampler in an urban location of Montreal, Quebec, Canada (45 degrees 28' N, 73 degrees 45' E). After isolation on Sabouraud dextrose agar, we exposed isolated colonies to dicarboxylic acids (C(2)-C(7)), a major group of organic aerosols and monitored their growth. Depending on the acid, total fungi numbers varied from 35 (oxalic acid) to 180 CFU/mL (glutaric acid). Transformation kinetics of malonic acid, presumably the most abundant dicarboxylic acid, at concentrations of 0.25 and 1.00 mM for isolated airborne fungi belonging to the genera Aspergillus, Penicillium, Eupenicillium, and Thysanophora with the fastest transformation rate are presented. The initial concentration was halved within 4.5 and 11.4 days. Other collected fungi did not show a significant degradation and the malonic acid concentration remained unchanged (0.25 and 1.00 mM) within 20 days. Degradation of acid with formation of metabolites was followed using high performance liquid chromatography-ultraviolet detection (HPLC/UV) and gas chromatography-mass spectrometry (GC/MS), as well as monitoring of (13)C labelled malonic acid degradation with solid-state nuclear magnetic resonance spectroscopy (NMR). Using GC/MS we identified two processes driving chemical modifications of organic aerosol solutions: (I) formation of metabolites within several days, and (II) rapid release (< or =2 min) of organic molecules from fungal species upon the insertion of taxa in organic aerosol solutions. Metabolites included aromatic compounds and alcohols (e.g. trimethylbenzene and butanol). Potential atmospheric implications of our results are discussed. |
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Authors:
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Valerié Côté; Gregor Kos; Roya Mortazavi; Parisa A Ariya |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2007-11-26 |
Journal Detail:
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Title: The Science of the total environment Volume: 390 ISSN: 0048-9697 ISO Abbreviation: Sci. Total Environ. Publication Date: 2008 Feb |
Date Detail:
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Created Date: 2007-12-21 Completed Date: 2008-04-08 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 0330500 Medline TA: Sci Total Environ Country: Netherlands |
Other Details:
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Languages: eng Pagination: 530-7 Citation Subset: IM |
Affiliation:
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McGill University, Department of Atmospheric and Oceanic Sciences, 801 Sherbrooke St. West, Montreal, Quebec, Canada H3A 2K6. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Air Microbiology* Air Pollutants / metabolism* Colony Count, Microbial DNA, Fungal / chemistry, genetics Dicarboxylic Acids / metabolism* Fungi / genetics, metabolism* Gas Chromatography-Mass Spectrometry Magnetic Resonance Spectroscopy Polymerase Chain Reaction Sequence Analysis, DNA |
| Chemical | |
Reg. No./Substance:
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0/Air Pollutants; 0/DNA, Fungal; 0/Dicarboxylic Acids |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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