Document Detail

Microbial surface interactions: reduction of the haemagglutination activity of the oral bacterium Fusobacterium nucleatum by absorption with Streptococcus and Bacteroides.
MedLine Citation:
PMID:  7041865     Owner:  NLM     Status:  MEDLINE    
Oral strains of Fusobacterium nucleatum showed haemagglutination (HA) of sheep red blood cells and attachment of HA-active F. nucleatum fragments to other microorganisms allowed a means of studying microbial surface interactions. HA-active sonicated fragments (SF) prepared from F. nucleatum were mixed with whole cell suspensions of 48 bacterial strains and, after incubation, the whole cells were separated from the non-absorbed fragments by differential centrifugation. Attachment of F. nucleatum fragments to the cells was indicated by a reduction in the HA activity of the SF in the supernatant fluid remaining after absorption with whole cells. HA activity of the microbial cells used for absorption and the detection of F. nucleatum fragments on these cells by an indirect fluorescent antibody technique provided further evidence of attachment. Of the 48 strains tested, 10 absorbed F. nucleatum HA-active fragments. They included Bacteroides gingivalis, Bacteroides fragilis subsp. distasonis, Bacteroides corrodens, Streptococcus morbillorum, Streptococcus sanguis (Blackburn and JC 74) and Streptococcus mutans AHT, BHT, 10449 and 6715. Chelators revealed that F. nucleatum attached to the microorganisms via a Ca2+-dependent interaction. Sugar inhibition demonstrated that F. nucleatum attached to the microorganisms via a D-galactose-containing moiety on their surface. A reduction in the absorption of F. nucleatum HA-active fragments by Strep. mutans grown in a higher concentration of sucrose was observed.
W A Falkler; B W Burger
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Archives of oral biology     Volume:  26     ISSN:  0003-9969     ISO Abbreviation:  Arch. Oral Biol.     Publication Date:  1981  
Date Detail:
Created Date:  1982-06-24     Completed Date:  1982-06-24     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0116711     Medline TA:  Arch Oral Biol     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  1015-25     Citation Subset:  D; IM    
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MeSH Terms
Bacteroides / metabolism*
Binding Sites
Chelating Agents
Fluorescent Antibody Technique
Fusobacterium / metabolism*
Streptococcus / metabolism*
Surface Properties
Reg. No./Substance:
0/Chelating Agents

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