Document Detail

Microbial production of glyceric acid, an organic acid that can be mass produced from glycerol.
MedLine Citation:
PMID:  19837846     Owner:  NLM     Status:  MEDLINE    
Glyceric acid (GA), an unfamiliar biotechnological product, is currently produced as a small by-product of dihydroxyacetone production from glycerol by Gluconobacter oxydans. We developed a method for the efficient biotechnological production of GA as a target compound for new surplus glycerol applications in the biodiesel and oleochemical industries. We investigated the ability of 162 acetic acid bacterial strains to produce GA from glycerol and found that the patterns of productivity and enantiomeric GA compositions obtained from several strains differed significantly. The growth parameters of two different strain types, Gluconobacter frateurii NBRC103465 and Acetobacter tropicalis NBRC16470, were optimized using a jar fermentor. G. frateurii accumulated 136.5 g/liter of GA with a 72% d-GA enantiomeric excess (ee) in the culture broth, whereas A. tropicalis produced 101.8 g/liter of d-GA with a 99% ee. The 136.5 g/liter of glycerate in the culture broth was concentrated to 236.5 g/liter by desalting electrodialysis during the 140-min operating time, and then, from 50 ml of the concentrated solution, 9.35 g of GA calcium salt was obtained by crystallization. Gene disruption analysis using G. oxydans IFO12528 revealed that the membrane-bound alcohol dehydrogenase (mADH)-encoding gene (adhA) is required for GA production, and purified mADH from G. oxydans IFO12528 catalyzed the oxidation of glycerol. These results strongly suggest that mADH is involved in GA production by acetic acid bacteria. We propose that GA is potentially mass producible from glycerol feedstock by a biotechnological process.
Hiroshi Habe; Yuko Shimada; Toshiharu Yakushi; Hiromi Hattori; Yoshitaka Ano; Tokuma Fukuoka; Dai Kitamoto; Masayuki Itagaki; Kunihiro Watanabe; Hiroshi Yanagishita; Kazunobu Matsushita; Keiji Sakaki
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2009-10-16
Journal Detail:
Title:  Applied and environmental microbiology     Volume:  75     ISSN:  1098-5336     ISO Abbreviation:  Appl. Environ. Microbiol.     Publication Date:  2009 Dec 
Date Detail:
Created Date:  2009-12-07     Completed Date:  2010-03-29     Revised Date:  2013-05-31    
Medline Journal Info:
Nlm Unique ID:  7605801     Medline TA:  Appl Environ Microbiol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  7760-6     Citation Subset:  IM    
Research Institute for Innovation in Sustainable Chemistry, National Institute of Advanced Industrial Science and Technology (AIST), Tsukuba Central 5-2, 1-1-1 Higashi, Tsukuba, Ibaraki 305-8565, Japan.
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MeSH Terms
Acetobacter / genetics,  metabolism
Alcohol Dehydrogenase / genetics,  metabolism
Dihydroxyacetone / metabolism
Gluconacetobacter / genetics,  metabolism
Gluconobacter / enzymology,  genetics,  metabolism*
Glyceric Acids / chemistry,  metabolism*
Glycerol / metabolism*
Industrial Microbiology / methods*
Reg. No./Substance:
0/Glyceric Acids; 56-81-5/Glycerol; 70KH64UX7G/glyceric acid; 96-26-4/Dihydroxyacetone; EC Dehydrogenase

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