Document Detail

Microbial production of R-3-hydroxybutyric acid by recombinant E. coli harboring genes of phbA, phbB, and tesB.
MedLine Citation:
PMID:  17609944     Owner:  NLM     Status:  MEDLINE    
Production of R-3-hydroxybutyric acid (3HB) was observed when genes of beta-ketothiolase (PhbA), acetoacetyl CoA reductase (PhbB), and thioesterase II (TesB) were jointly expressed in Escherichia coli. TesB, generally regarded as a medium chain length acyl CoA thioesterase, was found, for the first time, to play an important role for transforming short chain length 3-hydroxybutyrate-CoA to its free fatty acid, namely, 3HB. E. coli BW25113 (pSPB01) harboring phbA, phbB, and tesB genes produced approximately 4 g/l 3HB in shake flask culture within 24 h with glucose used as a carbon source. Under anaerobic growth conditions, 3HB production was found to be more effective, achieving 0.47 g 3HB/g glucose compared with only 0.32 g 3HB/g glucose obtained from aerobic process. When growth was conducted on sodium gluconate, 6 g/l 3HB was obtained. In a 24-h fed-batch growth process conducted in a 6-l fermentor containing 3 l glucose mineral medium, 12 g/l 3HB was produced from 17 g/l cell dry weight (CDW). This was the highest 3HB productivity achieved by a one-stage fermentation process for 3HB production.
Qian Liu; Shao-Ping Ouyang; Ahleum Chung; Qiong Wu; Guo-Qiang Chen
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2007-07-04
Journal Detail:
Title:  Applied microbiology and biotechnology     Volume:  76     ISSN:  0175-7598     ISO Abbreviation:  Appl. Microbiol. Biotechnol.     Publication Date:  2007 Sep 
Date Detail:
Created Date:  2007-08-29     Completed Date:  2007-11-13     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8406612     Medline TA:  Appl Microbiol Biotechnol     Country:  Germany    
Other Details:
Languages:  eng     Pagination:  811-8     Citation Subset:  IM    
Department of Biological Sciences and Biotechnology, Tsinghua University, Beijing, 100084, People's Republic of China.
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MeSH Terms
3-Hydroxybutyric Acid / metabolism*
Acetyl-CoA C-Acyltransferase / genetics,  metabolism*
Alcohol Oxidoreductases / genetics,  metabolism*
Biotechnology / methods*
Escherichia coli / genetics,  metabolism*
Fatty Acid Synthetase Complex / genetics,  metabolism*
Recombinant Proteins / biosynthesis,  genetics,  metabolism
Spectrophotometry, Ultraviolet
Thiolester Hydrolases / genetics,  metabolism*
Reg. No./Substance:
0/Recombinant Proteins; 300-85-6/3-Hydroxybutyric Acid; EC 1.1.-/Alcohol Oxidoreductases; EC reductase; EC C-Acyltransferase; EC 3.1.2.-/Thiolester Hydrolases; EC 3.1.2.-/thioesterase II; EC 6.-/Fatty Acid Synthetase Complex

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