Document Detail


Microbial metabolic exchange in 3D.
MedLine Citation:
PMID:  23283018     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Mono- and multispecies microbial populations alter the chemistry of their surrounding environments during colony development thereby influencing multicellular behavior and interspecies interactions of neighboring microbes. Here we present a methodology that enables the creation of three-dimensional (3D) models of a microbial chemotype that can be correlated to the colony phenotype through multimodal imaging analysis. These models are generated by performing matrix-assisted laser desorption ionization time-of-flight (MALDI-TOF) imaging mass spectrometry (IMS) on serial cross-sections of microbial colonies grown on 8 mm deep agar, registering data sets of each serial section in MATLAB to create a model, and then superimposing the model with a photograph of the colonies themselves. As proof-of-principle, 3D models were used to visualize metabolic exchange during microbial interactions between Bacillus subtilis and Streptomyces coelicolor, as well as, Candida albicans and Pseudomonas aeruginosa. The resulting models were able to capture the depth profile of secreted metabolites within the agar medium and revealed properties of certain mass signals that were previously not observable using two-dimensional MALDI-TOF IMS. Most significantly, the 3D models were capable of mapping previously unobserved chemical distributions within the array of sub-surface hyphae of C. albicans and how this chemistry is altered by the presence of P. aeruginosa, an opportunistic pathogen known to alter virulence of C. albicans. It was determined that the presence of C. albicans triggered increased rhamnolipid production by P. aeruginosa, which in turn was capable of inhibiting embedded hyphal growth produced beneath the C. albicans colony at ambient temperature.
Authors:
Jeramie D Watrous; Vanessa V Phelan; Cheng-Chih Hsu; Wilna J Moree; Brendan M Duggan; Theodore Alexandrov; Pieter C Dorrestein
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't     Date:  2013-01-03
Journal Detail:
Title:  The ISME journal     Volume:  7     ISSN:  1751-7370     ISO Abbreviation:  ISME J     Publication Date:  2013 Apr 
Date Detail:
Created Date:  2013-03-19     Completed Date:  2013-09-30     Revised Date:  2014-04-10    
Medline Journal Info:
Nlm Unique ID:  101301086     Medline TA:  ISME J     Country:  England    
Other Details:
Languages:  eng     Pagination:  770-80     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Bacillus subtilis / metabolism
Candida albicans / metabolism*
Culture Media / chemistry
Hyphae / metabolism
Imaging, Three-Dimensional*
Microbial Interactions*
Pseudomonas aeruginosa / metabolism*
Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / methods*
Streptomyces coelicolor / metabolism
Grant Support
ID/Acronym/Agency:
GM08326/GM/NIGMS NIH HHS; GM086283/GM/NIGMS NIH HHS; GM094802/GM/NIGMS NIH HHS; K01 GM103809/GM/NIGMS NIH HHS; R01 GM086283/GM/NIGMS NIH HHS; R01 GM094802/GM/NIGMS NIH HHS; T32 GM008326/GM/NIGMS NIH HHS
Chemical
Reg. No./Substance:
0/Culture Media
Comments/Corrections

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