Document Detail

Microbial invasion of the amniotic cavity in pregnancies with small-for-gestational-age fetuses.
MedLine Citation:
PMID:  20482466     Owner:  NLM     Status:  MEDLINE    
OBJECTIVE: Microbial invasion of the amniotic cavity (MIAC) has been detected in women with preterm labor, preterm prelabor rupture of membranes (PROM), and in patients at term with PROM or in spontaneous labor. Intrauterine infection is recognized as a potential cause of fetal growth restriction; yet, the frequency of MIAC in pregnancies with small-for-gestational-age (SGA) fetuses is unknown. The aim of this study was to determine the frequency, diversity and relative abundance of microbes in amniotic fluid (AF) of women with an SGA neonate using a combination of culture and molecular methods.
METHOD: AF from 52 subjects with an SGA neonate was analyzed with both cultivation and molecular methods in a retrospective cohort study. Broad-range and group-specific PCR assays targeted small subunit rDNA, or other gene sequences, from bacteria, fungi and archaea. Results of microbiologic studies were correlated with indices of the host inflammatory response.
RESULTS: 1) All AF samples (n=52) were negative for microorganisms based on cultivation techniques, whereas 6% (3/52) were positive based on PCR; and 2) intra-amniotic inflammation was detected in one of the three patients with a positive PCR result, as compared with three patients (6.1%) of the 49 with both a negative culture and a negative PCR (P=0.2).
CONCLUSION: MIAC is detected by PCR in some patients with an SGA fetus who were not in labor at the time of AF collection.
Daniel B DiGiulio; Maria Teresa Gervasi; Roberto Romero; Edi Vaisbuch; Shali Mazaki-Tovi; Juan Pedro Kusanovic; Kimberley S Seok; Ricardo Gómez; Pooja Mittal; Francesca Gotsch; Tinnakorn Chaiworapongsa; Enrique Oyarzún; Chong Jai Kim; David A Relman
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, N.I.H., Intramural; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Journal of perinatal medicine     Volume:  38     ISSN:  1619-3997     ISO Abbreviation:  J Perinat Med     Publication Date:  2010 Sep 
Date Detail:
Created Date:  2010-09-03     Completed Date:  2011-01-04     Revised Date:  2014-09-05    
Medline Journal Info:
Nlm Unique ID:  0361031     Medline TA:  J Perinat Med     Country:  Germany    
Other Details:
Languages:  eng     Pagination:  495-502     Citation Subset:  IM    
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MeSH Terms
Amnion / microbiology*
Amniotic Fluid / microbiology
Base Sequence
Chorioamnionitis / microbiology
Cohort Studies
DNA Primers / genetics
DNA, Archaeal / genetics,  isolation & purification
DNA, Bacterial / genetics,  isolation & purification
DNA, Fungal / genetics,  isolation & purification
Infant, Newborn
Infant, Small for Gestational Age*
Microbiological Techniques
Polymerase Chain Reaction
Pregnancy Complications, Infectious / microbiology*
Pregnancy Outcome
Retrospective Studies
Young Adult
Grant Support
DP1 OD000964/OD/NIH HHS; DP1 OD000964-03/OD/NIH HHS; DP1 OD000964-04/OD/NIH HHS; DP1OD000964/OD/NIH HHS; ZIA HD002400-18/HD/NICHD NIH HHS
Reg. No./Substance:
0/DNA Primers; 0/DNA, Archaeal; 0/DNA, Bacterial; 0/DNA, Fungal

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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