| Microbial invasion of the amniotic cavity in pregnancies with small-for-gestational-age fetuses. | |
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MedLine Citation:
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PMID: 20482466 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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OBJECTIVE: Microbial invasion of the amniotic cavity (MIAC) has been detected in women with preterm labor, preterm prelabor rupture of membranes (PROM), and in patients at term with PROM or in spontaneous labor. Intrauterine infection is recognized as a potential cause of fetal growth restriction; yet, the frequency of MIAC in pregnancies with small-for-gestational-age (SGA) fetuses is unknown. The aim of this study was to determine the frequency, diversity and relative abundance of microbes in amniotic fluid (AF) of women with an SGA neonate using a combination of culture and molecular methods. METHOD: AF from 52 subjects with an SGA neonate was analyzed with both cultivation and molecular methods in a retrospective cohort study. Broad-range and group-specific PCR assays targeted small subunit rDNA, or other gene sequences, from bacteria, fungi and archaea. Results of microbiologic studies were correlated with indices of the host inflammatory response. RESULTS: 1) All AF samples (n=52) were negative for microorganisms based on cultivation techniques, whereas 6% (3/52) were positive based on PCR; and 2) intra-amniotic inflammation was detected in one of the three patients with a positive PCR result, as compared with three patients (6.1%) of the 49 with both a negative culture and a negative PCR (P=0.2). CONCLUSION: MIAC is detected by PCR in some patients with an SGA fetus who were not in labor at the time of AF collection. |
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Authors:
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Daniel B DiGiulio; Maria Teresa Gervasi; Roberto Romero; Edi Vaisbuch; Shali Mazaki-Tovi; Juan Pedro Kusanovic; Kimberley S Seok; Ricardo Gómez; Pooja Mittal; Francesca Gotsch; Tinnakorn Chaiworapongsa; Enrique Oyarzún; Chong Jai Kim; David A Relman |
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Publication Detail:
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Type: Journal Article; Research Support, N.I.H., Extramural; Research Support, N.I.H., Intramural; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Journal of perinatal medicine Volume: 38 ISSN: 1619-3997 ISO Abbreviation: J Perinat Med Publication Date: 2010 Sep |
Date Detail:
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Created Date: 2010-09-03 Completed Date: 2011-01-04 Revised Date: 2011-06-30 |
Medline Journal Info:
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Nlm Unique ID: 0361031 Medline TA: J Perinat Med Country: Germany |
Other Details:
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Languages: eng Pagination: 495-502 Citation Subset: IM |
Affiliation:
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Department of Medicine, Stanford University School of Medicine, Stanford, CA, USA. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Adult Amnion / microbiology* Amniotic Fluid / microbiology Base Sequence Chorioamnionitis / microbiology Cohort Studies DNA Primers / genetics DNA, Archaeal / genetics, isolation & purification DNA, Bacterial / genetics, isolation & purification DNA, Fungal / genetics, isolation & purification Female Humans Infant, Newborn Infant, Small for Gestational Age* Microbiological Techniques Polymerase Chain Reaction Pregnancy Pregnancy Complications, Infectious / microbiology* Pregnancy Outcome Retrospective Studies Young Adult |
| Grant Support | |
ID/Acronym/Agency:
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DP1 OD000964-03/OD/NIH HHS; DP1 OD000964-04/OD/NIH HHS; DP1OD000964/OD/NIH HHS; ZIA HD002400-18/HD/NICHD NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/DNA Primers; 0/DNA, Archaeal; 0/DNA, Bacterial; 0/DNA, Fungal |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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