Document Detail


Microbial dynamics of co- and separately entrapped mixed cultures of mesophilic lactic acid bacteria during the continuous prefermentation of milk.
MedLine Citation:
PMID:  9084207     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Four strains of mesophilic lactic acid bacteria were separately or coentrapped in kappa-carrageenan/locust bean gum gel beads and used for continuous prefermentation of UHT skim milk in a stirred-tank bioreactor. Lactic acid and cell productivities of the immobilized cell bioreactor were particularly high and remarkably stable during eight weeks of continuous milk prefermentation (about 18 g h-1 l-1 of lactic acid and 4.9 x 10(12) CFU h-1 l-1, respectively, but important variations of the bacterial populations is prefermented milk and gel beads occurred in any case (co-or separate entrapment). The strain Lactococcus lactis subsp. lactis biovar diacetylactis CDII became dominant, accounting for approx. 90% (released cells) and 78% (immobilized cells) of the total population. Microscopic observations of sections of gels beads showed a progressive destructing of the bead surface with rupture and release of entrapped viable cells from peripheral cavities of the gel. It is believed that these cavities close again after releasing all or part of their cell content, entrapping the different strains of the mixed culture and initiating a new colonization step and a cross-contamination of the beads. On the other hand, experimentations over seven-week periods with pasteurized milk showed the high resistance of the immobilized cell bioreactor to psychrotrophic contamination.
Authors:
I Sodini; C Y Boquien; G Corrieu; C Lacroix
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Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Enzyme and microbial technology     Volume:  20     ISSN:  0141-0229     ISO Abbreviation:  Enzyme Microb. Technol.     Publication Date:  1997 Apr 
Date Detail:
Created Date:  1997-05-20     Completed Date:  1997-05-20     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  8003761     Medline TA:  Enzyme Microb Technol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  381-8     Citation Subset:  B    
Affiliation:
Laboratoire de Génie et de Microbiologie des Procédés Alimentaires, INRA, Thiverval-Grignon, France.
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MeSH Terms
Descriptor/Qualifier:
Animals
Bioreactors
Carrageenan / chemistry
Cells, Immobilized / ultrastructure
Colony Count, Microbial
Excipients / chemistry
Fermentation
Food Microbiology*
Galactans
Industrial Microbiology*
Lactococcus lactis / growth & development*,  metabolism
Mannans
Microspheres
Milk / microbiology*
Plant Gums
Polysaccharides / chemistry
Time Factors
Chemical
Reg. No./Substance:
0/Excipients; 0/Galactans; 0/Mannans; 0/Plant Gums; 0/Polysaccharides; 9000-07-1/Carrageenan; 9000-40-2/locust bean gum

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