Document Detail


Microbial domestication signatures of Lactococcus lactis can be reproduced by experimental evolution.
MedLine Citation:
PMID:  22080491     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Experimental evolution is a powerful approach to unravel how selective forces shape microbial genotypes and phenotypes. To this date, the available examples focus on the adaptation to conditions specific to the laboratory. The lactic acid bacterium Lactococcus lactis naturally occurs on plants and in dairy environments, and it is proposed that dairy strains originate from the plant niche. Here we investigate the adaptation of a L. lactis strain isolated from a plant to a dairy niche by propagating it for 1000 generations in milk. Two out of three independently evolved strains displayed significantly increased acidification rates and biomass yields in milk. Genome resequencing, revealed six, seven, and 28 mutations in the three strains, including point mutations in loci related to amino acid biosynthesis and transport and in the gene encoding MutL, which is involved in DNA mismatch repair. Two strains lost a conjugative transposon containing genes important in the plant niche but dispensable in milk. A plasmid carrying an extracellular protease was introduced by transformation. Although improving growth rate and growth yield significantly, the plasmid was rapidly lost. Comparative transcriptome and phenotypic analyses confirmed that major physiological changes associated with improved growth in milk relate to nitrogen metabolism and the loss or down-regulation of several pathways involved in the utilization of complex plant polymers. Reproducing the transition from the plant to the dairy niche through experimental evolution revealed several genome, transcriptome, and phenotype signatures that resemble those seen in strains isolated from either niche.
Authors:
Herwig Bachmann; Marjo J C Starrenburg; Douwe Molenaar; Michiel Kleerebezem; Johan E T van Hylckama Vlieg
Publication Detail:
Type:  Journal Article     Date:  2011-11-11
Journal Detail:
Title:  Genome research     Volume:  22     ISSN:  1549-5469     ISO Abbreviation:  Genome Res.     Publication Date:  2012 Jan 
Date Detail:
Created Date:  2012-01-04     Completed Date:  2012-04-24     Revised Date:  2013-06-27    
Medline Journal Info:
Nlm Unique ID:  9518021     Medline TA:  Genome Res     Country:  United States    
Other Details:
Languages:  eng     Pagination:  115-24     Citation Subset:  IM    
Affiliation:
NIZO Food Research, 6710BA Ede, The Netherlands.
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MeSH Terms
Descriptor/Qualifier:
Dairy Products / microbiology
Evolution, Molecular*
Gene Expression Regulation, Bacterial / physiology*
Genetic Loci / physiology*
Genome, Bacterial / physiology*
Lactococcus lactis / physiology*
Point Mutation
Transcription, Genetic / physiology*
Comments/Corrections

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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