Document Detail


Microbial community dynamics in anaerobic bioreactors and algal tanks treating piggery wastewater.
MedLine Citation:
PMID:  20349182     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Integrated biosystem is becoming a major aspect of wastewater management practice. Microbial communities in piggery wastewater sampled from anaerobic (thermophilic and mesophilic) and aerobic digesters (algal tanks) during waste remediation were analyzed by culture-independent techniques based on polymerase chain reaction-denaturing gradient gel electrophoresis (PCR-DGGE). The use of Muyzer's 314F-GC, 518R bacterial primers, and archaeal A934F, 1309R primers followed by partial 16s rDNA sequence analysis of the main bands from DGGE revealed the presence of unknown and as yet uncultured microorganisms but also showed functional and ecologically significant denitrifying, acetogenic bacteria along with autotrophic, hydrogenotrophic, and acetoclastic methanogen archaea. Thermophilic digesters were dominated by gamma-Proteobacteria, Methanothermobacter sp., while mesophilic digesters showed dominance by Firmicutes, uncultured bacteria, Methanosarcina, and Methanoculleus genera. Under aerobic conditions within algal tanks, pH rose from 7.17 to 9.32, with a significant decrease in total ammonia nitrogen, chemical oxygen demand, and soluble phosphorus levels. PCR-DGGE proved a useful tool for investigating the dynamics of microbial community in the bio-processing of piggery wastewater. Knowledge of the microbial communities involved in digestion of piggery wastewater will allow optimization of integrated biosystem by removing the main pollutants like inorganic ammonium-nitrogen, phosphorus, and pathogens from intensive farming system.
Authors:
Sayali S Patil; Martin S Kumar; Andrew S Ball
Publication Detail:
Type:  Journal Article     Date:  2010-03-27
Journal Detail:
Title:  Applied microbiology and biotechnology     Volume:  87     ISSN:  1432-0614     ISO Abbreviation:  Appl. Microbiol. Biotechnol.     Publication Date:  2010 Jun 
Date Detail:
Created Date:  2010-05-18     Completed Date:  2010-10-04     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8406612     Medline TA:  Appl Microbiol Biotechnol     Country:  Germany    
Other Details:
Languages:  eng     Pagination:  353-63     Citation Subset:  IM    
Affiliation:
School of Biological Sciences, Flinders University of South Australia, Bedford Park, Adelaide SA-5042, Australia. pati0018@flinders.edu.au
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MeSH Terms
Descriptor/Qualifier:
Algae / metabolism
Anaerobiosis
Animals
Archaea / classification,  genetics,  isolation & purification*,  metabolism
Bacteria / classification,  genetics,  isolation & purification*,  metabolism
Biodiversity*
Bioreactors / microbiology*
Medical Waste Disposal*
Molecular Sequence Data
Phylogeny
Sewage / microbiology*
Swine
Chemical
Reg. No./Substance:
0/Medical Waste Disposal; 0/Sewage

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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