Document Detail


Microbial biosurfactants and hydrolytic enzymes mediates in situ development of stable supra-molecular assemblies in fatty acids released from triglycerides.
MedLine Citation:
PMID:  20359870     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The present study demonstrates in situ formation of multilamellar stable vesicles (MLSVs) of fatty acids released during the growth of microorganisms in the presence of triglycerides. Release of lipase during initial phase of growth hydrolyzes the triglycerides and release free fatty acids (mono or diglycerides) and glycerol. By extending the growth and the prevailing composition of media (unspent nutrients, salts, pH of the medium, biosurfactants, fatty acids, glycerol) and agitation transforms free fatty acids to MLSV of both cylindrical and spherical macroscopic structures via micelle formation with in 240h of incubation. Cross-sectional view and SEM analysis of macroscopic structures reveal the existence of continuous multilayering. Thermo-gravimetric analysis illustrates the stability of the vesicles. FT-IR analysis emphasizes the presence of amide linkages, responsible for self-assembly processes. Schematic representation of formation of MLSV demonstrated for further understanding. Additional exploration on MLSV formation in arteries and the relationship between MLSV and in situ plaque formation by the components of blood in the arteries are schematically explained and submitted as supporting information (SI-2).
Authors:
Arumugam Gnanamani; V Kavitha; N Radhakrishnan; G Sekaran; G Suseela Rajakumar; A B Mandal
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2010-03-12
Journal Detail:
Title:  Colloids and surfaces. B, Biointerfaces     Volume:  78     ISSN:  1873-4367     ISO Abbreviation:  Colloids Surf B Biointerfaces     Publication Date:  2010 Jul 
Date Detail:
Created Date:  2010-05-03     Completed Date:  2010-08-20     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9315133     Medline TA:  Colloids Surf B Biointerfaces     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  200-7     Citation Subset:  IM    
Copyright Information:
2010 Elsevier B.V. All rights reserved.
Affiliation:
Microbiology Division, Central Leather Research Institute, (CSIR, New Delhi), Adyar, Chennai 20, Tamil Nadu, India. agmani_2000@yahoo.com
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:
Bacillus / chemistry*,  metabolism,  ultrastructure
Bacterial Proteins / metabolism
Fatty Acids, Nonesterified / chemistry*,  metabolism
Hydrogen-Ion Concentration
Hydrolysis
Lipase / metabolism
Liposomes / chemistry,  metabolism,  ultrastructure
Microscopy, Electron, Scanning
Spectroscopy, Fourier Transform Infrared
Surface-Active Agents / chemistry*,  metabolism
Thermogravimetry
Triglycerides / chemistry*,  metabolism
Chemical
Reg. No./Substance:
0/Bacterial Proteins; 0/Fatty Acids, Nonesterified; 0/Liposomes; 0/Surface-Active Agents; 0/Triglycerides; EC 3.1.1.3/Lipase

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Molecular characteristics of extended-spectrum beta-lactamase-producing Escherichia coli from the Ch...
Next Document:  Protective effect of date palm fruit extract (Phoenix dactylifera L.) on dimethoate induced-oxidativ...