Document Detail

Microbial bioburden in endoscope reprocessing and an in-use evaluation of the high-level disinfection capabilities of Cidex PA.
MedLine Citation:
PMID:  10382415     Owner:  NLM     Status:  MEDLINE    
Most endoscopy clinics use 2% glutaraldehyde as a high-level disinfectant for reprocessing flexible endoscopes. However, even with contact times greater than 30 minutes, survival of organisms has been documented. We compared the high-level disinfection capabilities of glutaraldehyde (45-minute immersion) with a new peracetic acid germicide (20- to 25-minute immersion). Channels, valve housings, and outer sheaths were sampled to quantify bioburden levels after a patient procedure, after manual cleaning, and after disinfection. Total mean bioburden after clinical use was greater than 6 log10 colony-forming units (CFU). Manual cleaning reduced the bioburden by means of 4.7 log10 CFU (gastroscopes) and 6.2 log10 CFU (colonoscopes). High-level disinfection with the new product was achieved in five of six (product stressed by EPA Reuse Test) and 7 of 10 (product stressed by dilution and organic load) successfully disinfected endoscopes, whereas glutaraldehyde achieved it in 4 of 10 (product stressed by dilution and organic load). We conclude that the new peracetic acid product (20- and 25-minute contact time) is at least as effective as glutaraldehyde (45-minute contact time) for reducing the bioburden of vegetative aerobic organisms in endoscopes.
D Vesley; J Melson; P Stanley
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Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Gastroenterology nursing : the official journal of the Society of Gastroenterology Nurses and Associates     Volume:  22     ISSN:  1042-895X     ISO Abbreviation:  Gastroenterol Nurs     Publication Date:    1999 Mar-Apr
Date Detail:
Created Date:  2000-07-06     Completed Date:  2000-07-06     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  8915377     Medline TA:  Gastroenterol Nurs     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  63-8     Citation Subset:  N    
Division of Environmental and Occupational Health, School of Public Health, University of Minnesota, Minneapolis 55455, USA.
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MeSH Terms
Colony Count, Microbial
Cross Infection / prevention & control
Disinfection / methods*
Drug Combinations
Drug Evaluation, Preclinical
Endoscopes / microbiology*
Endoscopy / nursing
Equipment Contamination / prevention & control*
Peracetic Acid*
Time Factors
Reg. No./Substance:
0/Disinfectants; 0/Drug Combinations; 111-30-8/Glutaral; 79-21-0/Peracetic Acid
Comment In:
Gastroenterol Nurs. 1999 Mar-Apr;22(2):62   [PMID:  10382414 ]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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