| Microbial stimulation fully differentiates monocytes to DC-SIGN/CD209(+) dendritic cells for immune T cell areas. | |
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MedLine Citation:
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PMID: 21029863 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Dendritic cells (DCs), critical antigen-presenting cells for immune control, normally derive from bone marrow precursors distinct from monocytes. It is not yet established if the large reservoir of monocytes can develop into cells with critical features of DCs in vivo. We now show that fully differentiated monocyte-derived DCs (Mo-DCs) develop in mice and DC-SIGN/CD209a marks the cells. Mo-DCs are recruited from blood monocytes into lymph nodes by lipopolysaccharide and live or dead gram-negative bacteria. Mobilization requires TLR4 and its CD14 coreceptor and Trif. When tested for antigen-presenting function, Mo-DCs are as active as classical DCs, including cross-presentation of proteins and live gram-negative bacteria on MHC I in vivo. Fully differentiated Mo-DCs acquire DC morphology and localize to T cell areas via L-selectin and CCR7. Thus the blood monocyte reservoir becomes the dominant presenting cell in response to select microbes, yielding DC-SIGN(+) cells with critical functions of DCs. |
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Authors:
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Cheolho Cheong; Ines Matos; Jae-Hoon Choi; Durga Bhavani Dandamudi; Elina Shrestha; M Paula Longhi; Kate L Jeffrey; Robert M Anthony; Courtney Kluger; Godwin Nchinda; Hyein Koh; Anthony Rodriguez; Juliana Idoyaga; Maggi Pack; Klara Velinzon; Chae Gyu Park; Ralph M Steinman |
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Publication Detail:
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Type: Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Cell Volume: 143 ISSN: 1097-4172 ISO Abbreviation: Cell Publication Date: 2010 Oct |
Date Detail:
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Created Date: 2010-10-29 Completed Date: 2010-11-22 Revised Date: 2011-10-31 |
Medline Journal Info:
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Nlm Unique ID: 0413066 Medline TA: Cell Country: United States |
Other Details:
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Languages: eng Pagination: 416-29 Citation Subset: IM |
Copyright Information:
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Copyright © 2010 Elsevier Inc. All rights reserved. |
Affiliation:
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Chris Browne Center for Immunology and Immune Diseases, The Rockefeller University, New York, NY 10065, USA. ccheong@rockefeller.edu |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Antigen Presentation Antigens, CD14 / immunology Cell Adhesion Molecules / immunology, metabolism* Cell Differentiation* Dendritic Cells / cytology*, immunology Escherichia coli / immunology* L-Selectin / immunology Lectins, C-Type / immunology, metabolism* Lymph Nodes / cytology, immunology Mice Mice, Inbred BALB C Mice, Inbred C57BL Monocytes / cytology*, immunology Receptors, CCR7 / immunology Receptors, Cell Surface / immunology, metabolism* T-Lymphocytes / immunology Toll-Like Receptor 4 / agonists, immunology |
| Grant Support | |
ID/Acronym/Agency:
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AI40045/AI/NIAID NIH HHS; AI40874/AI/NIAID NIH HHS; GM62116/GM/NIGMS NIH HHS; R01 AI040874-13A1/AI/NIAID NIH HHS; R37 AI040045-15/AI/NIAID NIH HHS; UL1RR024143/RR/NCRR NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Antigens, CD14; 0/Ccr7 protein, mouse; 0/Cell Adhesion Molecules; 0/DC-specific ICAM-3 grabbing nonintegrin; 0/Lectins, C-Type; 0/Receptors, CCR7; 0/Receptors, Cell Surface; 0/Toll-Like Receptor 4; 126880-86-2/L-Selectin |
| Comments/Corrections | |
Comment In:
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Cell. 2010 Oct 29;143(3):339-40
[PMID:
21029856
]
Nat Rev Immunol. 2010 Dec;10(12):808 [PMID: 21155170 ] |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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