| Microbial Production of Propionic Acid with Propionibacterium freudenreichii Using an Anion Exchanger-Based In Situ Product Recovery (ISPR) Process with Direct and Indirect Contact of Cells. | |
| | |
MedLine Citation:
|
PMID: 22194053 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
|
The recovery of an inhibiting product from a bioreactor soon after its formation is an important issue in industrial bioprocess development. In the present study, the potential of the anion exchanger-based in situ product recovery (ISPR) technique for the biocatalytic production of propionic acid was discussed. The focus of the current work was the selection of a suitable configuration of metabolically active cells for application in propionic acid production. Accumulation of propionic acid in fermentation broth caused feedback inhibition of the growth and biotransformation activity of Propionibacterium freudenreichii CICC 10019. Relevant product inhibition kinetics was discussed, and the results showed that keeping the aqueous propionic acid concentration below 10.02 g L(-1) was an essential prerequisite for ISPR process. A batch study, in which three ISPR configuration mode designs were compared, was conducted. The comparison indicated that employing an external direct mode had significant advantages over other modes in terms of increased productivity and product yield, with a corresponding decrease in the number of downstream processing steps, as well as in substrate consumption. The fed-batch culture using an external direct mode for the continuous accumulation of propionic acid resulted in a cumulative propionic acid concentration of 62.5 g L(-1), with a corresponding product yield of 0.78 g propionic acid/g glucose. |
| | |
Authors:
|
Peng Wang; Yunshan Wang; Zhiguo Su |
Related Documents
:
|
163783 - Induction of nutritional mutants of micrococcus glutamicus and their amino acid accumul... 8558593 - Spatial organization of transport domains and subdomain formation in the plasma membran... 1938913 - Multiple domains in endoglucanase b (cenb) from cellulomonas fimi: functions and relate... |
Publication Detail:
|
Type: JOURNAL ARTICLE Date: 2011-12-23 |
Journal Detail:
|
Title: Applied biochemistry and biotechnology Volume: - ISSN: 1559-0291 ISO Abbreviation: - Publication Date: 2011 Dec |
Date Detail:
|
Created Date: 2011-12-23 Completed Date: - Revised Date: - |
Medline Journal Info:
|
Nlm Unique ID: 8208561 Medline TA: Appl Biochem Biotechnol Country: - |
Other Details:
|
Languages: ENG Pagination: - Citation Subset: - |
Affiliation:
|
National Key Laboratory of Biochemical Engineering, Institute of Process Engineering, Chinese Academy of Sciences, P.O. Box 353, Beijing, 100190, People's Republic of China. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
|
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Apoptosis Mechanism of Human Cholangiocarcinoma Cells Induced by Bile Extract from Crocodile.
Next Document: Role of Tyr-435 of Vibrio harveyi Chitinase A in Chitin Utilization.