Document Detail


Microbial diversity in anaerobic sediments at Rio Tinto, a naturally acidic environment with a high heavy metal content.
MedLine Citation:
PMID:  21724883     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The Tinto River is an extreme environment located at the core of the Iberian Pyritic Belt (IPB). It is an unusual ecosystem due to its size (100 km long), constant acidic pH (mean pH, 2.3), and high concentration of heavy metals, iron, and sulfate in its waters, characteristics that make the Tinto River Basin comparable to acidic mine drainage (AMD) systems. In this paper we present an extensive survey of the Tinto River sediment microbiota using two culture-independent approaches: denaturing gradient gel electrophoresis and cloning of 16S rRNA genes. The taxonomic affiliation of the Bacteria showed a high degree of biodiversity, falling into 5 different phyla: Proteobacteria, Firmicutes, Bacteroidetes, Acidobacteria, and Actinobacteria; meanwhile, all the Archaea were affiliated with the order Thermoplasmatales. Microorganisms involved in the iron (Acidithiobacillus ferrooxidans, Sulfobacillus spp., Ferroplasma spp., etc.), sulfur (Desulfurella spp., Desulfosporosinus spp., Thermodesulfobium spp., etc.), and carbon (Acidiphilium spp., Bacillus spp., Clostridium spp., Acidobacterium spp., etc.) cycles were identified, and their distribution was correlated with physicochemical parameters of the sediments. Ferric iron was the main electron acceptor for the oxidation of organic matter in the most acid and oxidizing layers, so acidophilic facultative Fe(III)-reducing bacteria appeared widely in the clone libraries. With increasing pH, the solubility of iron decreases and sulfate-reducing bacteria become dominant, with the ecological role of methanogens being insignificant. Considering the identified microorganisms-which, according to the rarefaction curves and Good's coverage values, cover almost all of the diversity-and their corresponding metabolism, we suggest a model of the iron, sulfur, and organic matter cycles in AMD-related sediments.
Authors:
Irene Sánchez-Andrea; Nuria Rodríguez; Ricardo Amils; José Luis Sanz
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2011-07-01
Journal Detail:
Title:  Applied and environmental microbiology     Volume:  77     ISSN:  1098-5336     ISO Abbreviation:  Appl. Environ. Microbiol.     Publication Date:  2011 Sep 
Date Detail:
Created Date:  2011-08-25     Completed Date:  2011-12-13     Revised Date:  2013-06-28    
Medline Journal Info:
Nlm Unique ID:  7605801     Medline TA:  Appl Environ Microbiol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  6085-93     Citation Subset:  IM    
Affiliation:
Departamento de Biología Molecular, Universidad Autónoma de Madrid, c/Darwin 2, C-014/021, Campus de Cantoblanco, Crtra. de Colmenar, Km. 15, E-28049 Madrid, Spain.
Data Bank Information
Bank Name/Acc. No.:
GENBANK/HQ730609;  HQ730610;  HQ730611;  HQ730612;  HQ730613;  HQ730614;  HQ730615;  HQ730616;  HQ730617;  HQ730618;  HQ730619;  HQ730620;  HQ730621;  HQ730622;  HQ730623;  HQ730624;  HQ730625;  HQ730626;  HQ730627;  HQ730628;  HQ730629;  HQ730630;  HQ730631;  HQ730632;  HQ730633;  HQ730634;  HQ730635;  HQ730636;  HQ730637;  HQ730638;  HQ730639;  HQ730640;  HQ730641;  HQ730642;  HQ730643;  HQ730644;  HQ730645;  HQ730646;  HQ730647;  HQ730648;  HQ730649;  HQ730650;  HQ730651;  HQ730652;  HQ730653;  HQ730654;  HQ730655;  HQ730656;  HQ730657;  HQ730658;  HQ730659;  HQ730660;  HQ730661;  HQ730662;  HQ730663;  HQ730664;  HQ730665;  HQ730666;  HQ730667;  HQ730668;  HQ730669;  HQ730670;  HQ730671;  HQ730672;  HQ730673;  HQ730674;  HQ730675;  HQ730676;  HQ730677;  HQ730678;  HQ730679;  HQ730680;  HQ730681;  HQ730682;  HQ730683;  HQ730684;  HQ730685;  HQ730686;  HQ730687;  HQ730688;  HQ730689;  HQ730690;  HQ730691;  HQ730692;  HQ730693;  HQ730694;  HQ730695;  HQ730696;  HQ730697;  HQ730698;  HQ730699;  HQ730700;  HQ730701;  HQ730702;  HQ730703;  HQ730704;  HQ730705;  HQ730706;  HQ730707;  HQ730708;  HQ730709;  HQ730710;  HQ730711;  HQ730712;  HQ730713;  HQ730714;  HQ730715;  HQ730716;  HQ730717;  HQ730718;  HQ730719;  HQ730720;  HQ730721;  HQ730722;  HQ730723;  HQ730724;  HQ730725;  HQ730726;  HQ730727;  HQ730728;  HQ730729;  HQ730730;  HQ730731;  HQ730732;  HQ730733;  HQ730734;  HQ730735;  HQ730736;  HQ730737;  HQ730738;  HQ730739;  HQ730740;  HQ730741;  HQ730742;  HQ730743;  HQ730744;  HQ730745;  HQ730746;  HQ730747;  HQ730748;  HQ730749;  HQ730750;  HQ730751;  HQ730752;  HQ730753;  HQ730754;  HQ730755;  HQ730756;  HQ853235;  HQ853236;  HQ916664;  HQ916665;  HQ916666;  HQ916667
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MeSH Terms
Descriptor/Qualifier:
Archaea / classification*,  genetics*
Bacteria / classification*,  genetics*
Biodiversity*
Cloning, Molecular
Cluster Analysis
DNA, Archaeal / chemistry,  genetics
DNA, Bacterial / chemistry,  genetics
DNA, Ribosomal / chemistry,  genetics
Denaturing Gradient Gel Electrophoresis
Genes, rRNA
Geologic Sediments / microbiology*
Molecular Sequence Data
Phylogeny
RNA, Archaeal / genetics
RNA, Bacterial / genetics
RNA, Ribosomal, 16S / genetics
Rivers
Sequence Analysis, DNA
Sequence Homology, Nucleic Acid
Spain
Water Microbiology*
Chemical
Reg. No./Substance:
0/DNA, Archaeal; 0/DNA, Bacterial; 0/DNA, Ribosomal; 0/RNA, Archaeal; 0/RNA, Bacterial; 0/RNA, Ribosomal, 16S
Comments/Corrections

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