Document Detail


Succession of microbial consortia in the developing infant gut microbiome.
MedLine Citation:
PMID:  20668239     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The colonization process of the infant gut microbiome has been called chaotic, but this view could reflect insufficient documentation of the factors affecting the microbiome. We performed a 2.5-y case study of the assembly of the human infant gut microbiome, to relate life events to microbiome composition and function. Sixty fecal samples were collected from a healthy infant along with a diary of diet and health status. Analysis of >300,000 16S rRNA genes indicated that the phylogenetic diversity of the microbiome increased gradually over time and that changes in community composition conformed to a smooth temporal gradient. In contrast, major taxonomic groups showed abrupt shifts in abundance corresponding to changes in diet or health. Community assembly was nonrandom: we observed discrete steps of bacterial succession punctuated by life events. Furthermore, analysis of ≈ 500,000 DNA metagenomic reads from 12 fecal samples revealed that the earliest microbiome was enriched in genes facilitating lactate utilization, and that functional genes involved in plant polysaccharide metabolism were present before the introduction of solid food, priming the infant gut for an adult diet. However, ingestion of table foods caused a sustained increase in the abundance of Bacteroidetes, elevated fecal short chain fatty acid levels, enrichment of genes associated with carbohydrate utilization, vitamin biosynthesis, and xenobiotic degradation, and a more stable community composition, all of which are characteristic of the adult microbiome. This study revealed that seemingly chaotic shifts in the microbiome are associated with life events; however, additional experiments ought to be conducted to assess how different infants respond to similar life events.
Authors:
Jeremy E Koenig; Aymé Spor; Nicholas Scalfone; Ashwana D Fricker; Jesse Stombaugh; Rob Knight; Largus T Angenent; Ruth E Ley
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't     Date:  2010-07-28
Journal Detail:
Title:  Proceedings of the National Academy of Sciences of the United States of America     Volume:  108 Suppl 1     ISSN:  1091-6490     ISO Abbreviation:  Proc. Natl. Acad. Sci. U.S.A.     Publication Date:  2011 Mar 
Date Detail:
Created Date:  2011-03-16     Completed Date:  2011-05-17     Revised Date:  2012-04-26    
Medline Journal Info:
Nlm Unique ID:  7505876     Medline TA:  Proc Natl Acad Sci U S A     Country:  United States    
Other Details:
Languages:  eng     Pagination:  4578-85     Citation Subset:  IM    
Affiliation:
Department of Microbiology, Cornell University, Ithaca, NY 14853, USA.
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MeSH Terms
Descriptor/Qualifier:
Age Factors
Base Sequence
Cluster Analysis
DNA Barcoding, Taxonomic
DNA Primers / genetics
Feces / microbiology
Gastrointestinal Tract / microbiology*
Humans
Infant
Infant, Newborn
Metagenome / genetics*
Molecular Sequence Data
RNA, Ribosomal, 16S / genetics
Sequence Analysis, DNA
Species Specificity
Chemical
Reg. No./Substance:
0/DNA Primers; 0/RNA, Ribosomal, 16S
Comments/Corrections

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