| Microarrays for universal detection and identification of phytoplasmas. | |
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MedLine Citation:
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PMID: 22987419 Owner: NLM Status: In-Data-Review |
Abstract/OtherAbstract:
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Detection and identification of phytoplasmas is a laborious process often involving nested PCR followed by restriction enzyme analysis and fine-resolution gel electrophoresis. To improve throughput, other methods are needed. Microarray technology offers a generic assay that can potentially detect and differentiate all types of phytoplasmas in one assay. The present protocol describes a microarray-based method for identification of phytoplasmas to 16Sr group level. |
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Authors:
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Mogens Nicolaisen; Henriette Nyskjold; Assunta Bertaccini |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Methods in molecular biology (Clifton, N.J.) Volume: 938 ISSN: 1940-6029 ISO Abbreviation: Methods Mol. Biol. Publication Date: 2013 |
Date Detail:
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Created Date: 2012-09-18 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 9214969 Medline TA: Methods Mol Biol Country: United States |
Other Details:
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Languages: eng Pagination: 223-32 Citation Subset: IM |
Affiliation:
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Department of Agroecology, Aarhus University, Slagelse, Denmark, mogens.nicolaisen@agrsci.dk. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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