Document Detail


Microarrays for the study of viral gene expression during human cytomegalovirus latent infection.
MedLine Citation:
PMID:  18453089     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Human cytomegalovirus (HCMV) is one of the largest known DNA viruses. It is ubiquitous, and following resolution of primary productive infection, it persists in the human host by establishing a lifelong latent infection in myeloid lineage cells such as monocytes and their progenitors. Most adults with HCMV infection are healthy but it can cause neurologic deficits in infants, and remains an important cause of morbidity and mortality in the immunosuppressed patient. Microarray-based studies of HCMV have provided useful information about genes that are transcriptionally active during both productive and latent phases of infection. This chapter describes how to study genes in HCMV using microarrays and two cell types (productively infected human foreskin fibroblasts, and latently infected primary human myeloid progenitor cells).
Authors:
Barry Slobedman; Allen K L Cheung
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Methods in molecular medicine     Volume:  141     ISSN:  1543-1894     ISO Abbreviation:  Methods Mol. Med.     Publication Date:  2008  
Date Detail:
Created Date:  2008-05-05     Completed Date:  2008-06-20     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  101123138     Medline TA:  Methods Mol Med     Country:  United States    
Other Details:
Languages:  eng     Pagination:  153-75     Citation Subset:  IM    
Affiliation:
Centre for Virus Research, Westmead Millennium Institute, Westmead, New South Wales, Australia.
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MeSH Terms
Descriptor/Qualifier:
Cells, Cultured
Cytomegalovirus Infections / genetics*
DNA, Viral / isolation & purification
Gene Expression Profiling / methods*
Gene Expression Regulation, Viral*
Genes, Viral
Humans
Nucleic Acid Amplification Techniques
Nucleic Acid Hybridization / methods
Oligonucleotide Array Sequence Analysis / methods*
RNA / chemistry,  isolation & purification
Staining and Labeling / methods
Validation Studies as Topic
Virus Latency / genetics*
Chemical
Reg. No./Substance:
0/DNA, Viral; 63231-63-0/RNA

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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