Document Detail


Microarray phenotyping in Dictyostelium reveals a regulon of chemotaxis genes.
MedLine Citation:
PMID:  16234315     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
MOTIVATION: Coordinate regulation of gene expression can provide information on gene function. To begin a large-scale analysis of Dictyostelium gene function, we clustered genes based on their expression in wild-type and mutant strains and analyzed their functions.
RESULTS: We found 17 modes of wild-type gene expression and refined them into 57 submodes considering mutant data. Annotation analyses revealed correlations between co-expression and function and an unexpected correlation between expression and function of genes involved in various aspects of chemotaxis. Co-regulation of chemotaxis genes was also found in published data from neutrophils. To test the predictive power of the analysis, we examined the phenotypes of mutations in seven co-regulated genes that had no published role in chemotaxis. Six mutants exhibited chemotaxis defects, supporting the idea that function can be inferred from co-expression. The clustering and annotation analyses provide a public resource for Dictyostelium functional genomics.
Authors:
Ezgi O Booth; Nancy Van Driessche; Olga Zhuchenko; Adam Kuspa; Gad Shaulsky
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't     Date:  2005-10-18
Journal Detail:
Title:  Bioinformatics (Oxford, England)     Volume:  21     ISSN:  1367-4803     ISO Abbreviation:  Bioinformatics     Publication Date:  2005 Dec 
Date Detail:
Created Date:  2005-12-07     Completed Date:  2006-02-27     Revised Date:  2013-05-20    
Medline Journal Info:
Nlm Unique ID:  9808944     Medline TA:  Bioinformatics     Country:  England    
Other Details:
Languages:  eng     Pagination:  4371-7     Citation Subset:  IM    
Affiliation:
Graduate Program in Structural and Computational Biology and Molecular Biophysics, Baylor College of Medicine, Houston, TX 77030, USA.
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MeSH Terms
Descriptor/Qualifier:
Animals
Chemotaxis / drug effects,  genetics*
Cluster Analysis
Computational Biology
Cyclic AMP / pharmacology
Cytoskeleton / genetics
Dictyostelium / drug effects,  genetics*,  physiology
Gene Expression Profiling / statistics & numerical data
Gene Expression Regulation
Genes, Protozoan*
Genomics / statistics & numerical data
Oligonucleotide Array Sequence Analysis / statistics & numerical data
Phenotype
Regulon
Ribosomes / genetics
Signal Transduction
Grant Support
ID/Acronym/Agency:
P01 HD39691/HD/NICHD NIH HHS
Chemical
Reg. No./Substance:
60-92-4/Cyclic AMP

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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