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Microarray expression profiling in adhesion and normal peritoneal tissues.
MedLine Citation:
PMID:  22365381     Owner:  NLM     Status:  Publisher    
OBJECTIVE: To identify molecular markers associated with adhesion and normal peritoneal tissue using microarray expression profiling. DESIGN: Comparative study. SETTING: University hospital. PATIENT(S): Five premenopausal women. INTERVENTION(S): Adhesion and normal peritoneal tissue samples were obtained from premenopausal women. Ribonucleic acid was extracted using standard protocols and processed for hybridization to Affymetrix Whole Transcript Human Gene Expression Chips. Microarray data were obtained from five different patients, each with adhesion tissue and normal peritoneal samples. Real-time polymerase chain reaction was performed for confirmation using standard protocols. MAIN OUTCOME MEASURE(S): Gene expression in postoperative adhesion and normal peritoneal tissues. RESULT(S): A total of 1,263 genes were differentially expressed between adhesion and normal tissues. One hundred seventy-three genes were found to be up-regulated and 56 genes were down-regulated in the adhesion tissues compared with normal peritoneal tissues. The genes were sorted into functional categories according to Gene Ontology annotations. Twenty-six up-regulated genes and 11 down-regulated genes were identified with functions potentially relevant to the pathophysiology of postoperative adhesions. We evaluated and confirmed expression of 12 of these specific genes via polymerase chain reaction. CONCLUSION(S): The pathogenesis, natural history, and optimal treatment of postoperative adhesive disease remains unanswered. Microarray analysis of adhesions identified specific genes with increased and decreased expression when compared with normal peritoneum. Knowledge of these genes and ontologic pathways with altered expression provide targets for new therapies to treat patients who have or are at risk for postoperative adhesions.
Dana R Ambler; Alicia M Golden; Jennifer S Gell; Ghassan M Saed; David J Carey; Michael P Diamond
Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2012-2-22
Journal Detail:
Title:  Fertility and sterility     Volume:  -     ISSN:  1556-5653     ISO Abbreviation:  -     Publication Date:  2012 Feb 
Date Detail:
Created Date:  2012-2-27     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0372772     Medline TA:  Fertil Steril     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Copyright Information:
Copyright © 2012 American Society for Reproductive Medicine. Published by Elsevier Inc. All rights reserved.
Division of Reproductive Endocrinology and Infertility, Department of Obstetrics and Gynecology, Geisinger Medical Center, Danville, Pennsylvania.
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