Document Detail


Microarray analysis of BeWo and JEG3 trophoblast cell lines: identification of differentially expressed transcripts.
MedLine Citation:
PMID:  16797695     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Trophoblast cell lines are important research tools used as a surrogate for primary trophoblast cells in the study of placental function. Because the cellular origins of transformed trophoblasts are likely to be diverse, it would be of value to understand the unique and shared phenotypes of the cells on a global scale. We have compared two widely used cell lines, BeWo and JEG3, by microarray analysis in order to identify differentially expressed genes. Results indicated that approximately 2700 genes were differentially expressed between the cell lines, with principal differences observed in the biological processes of response to stress, cell adhesion, signal transduction, and protein and nucleobase metabolisms. These data suggest that BeWo and JEG3 cell lines, and perhaps other trophoblast cell lines, are sufficiently dissimilar from each other such that they will be differentially suited for specific experimental paradigms.
Authors:
D W Burleigh; C M Kendziorski; Y J Choi; K M Grindle; R L Grendell; R R Magness; T G Golos
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural     Date:  2006-06-23
Journal Detail:
Title:  Placenta     Volume:  28     ISSN:  0143-4004     ISO Abbreviation:  Placenta     Publication Date:    2007 May-Jun
Date Detail:
Created Date:  2007-04-24     Completed Date:  2007-08-01     Revised Date:  2007-12-03    
Medline Journal Info:
Nlm Unique ID:  8006349     Medline TA:  Placenta     Country:  England    
Other Details:
Languages:  eng     Pagination:  383-9     Citation Subset:  IM    
Affiliation:
Wisconsin National Primate Research Center, University of Wisconsin Medical School, 1220 Capitol Court, Madison, WI 53715-1299, USA.
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MeSH Terms
Descriptor/Qualifier:
Cell Line
DNA Primers
Female
Gene Expression Regulation, Developmental*
Humans
Integrins / genetics
Oligonucleotide Array Sequence Analysis*
Placenta / cytology,  physiology
Polymerase Chain Reaction
Pregnancy
Proteins / genetics
Transcription, Genetic
Trophoblasts / cytology*,  physiology*
Grant Support
ID/Acronym/Agency:
P01 HD38843/HD/NICHD NIH HHS; P51 RR000167/RR/NCRR NIH HHS; R01 HD37120/HD/NICHD NIH HHS
Chemical
Reg. No./Substance:
0/DNA Primers; 0/Integrins; 0/Proteins

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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