Document Detail

MicroRNAs 29 are involved in the improvement of ventricular compliance promoted by aerobic exercise training in rats.
MedLine Citation:
PMID:  21447748     Owner:  NLM     Status:  MEDLINE    
MiRNAs regulate cardiac development, hypertrophy, and angiogenesis, but their role in cardiac hypertrophy (CH) induced by aerobic training has not previously been studied. Aerobic training promotes physiological CH preserving cardiac function. This study assessed involvement of miRNAs-29 in CH of trained rats. Female Wistar rats (n=7/group) were randomized into three groups: sedentary (S), training 1 (T1), training 2 (T2). T1: swimming sessions of 60 min/5 days/wk/10 wk. T2: similar to T1 until 8th wk. On the 9th wk rats swam 2×/day, and on the 10th wk 3×/day. MiRNAs analysis was performed by miRNA microarray and confirmed by real-time PCR. We assessed: markers of training, CH by ratio of left ventricle (LV) weight/body wt and cardiomyocytes diameter, pathological markers of CH (ANF, skeletal α-actin, α/β-MHC), collagen I and III (COLIAI and COLIIIAI) by real-time PCR, protein collagen by hydroxyproline (OH-proline) concentration, CF and CH by echocardiography. Training improved aerobic capacity and induced CH. MiRNAs-1, 133a, and 133b were downregulated as observed in pathological CH, however, without pathological markers. MiRNA-29c expression increased in T1 (52%) and T2 (123%), correlated with a decrease in COLIAI and COLIIIAI expression in T1 (27%, 38%) and T2 (33%, 48%), respectively. MiRNA-29c was inversely correlated to OH-proline concentration (r=0.61, P<0.05). The E/A ratio increased in T2, indicating improved LV compliance. Thus, these results show that aerobic training increase miR-29 expression and decreased collagen gene expression and concentration in the heart, which is relevant to the improved LV compliance and beneficial cardiac effects, associated with aerobic high performance training.
U P R Soci; T Fernandes; N Y Hashimoto; G F Mota; M A Amadeu; K T Rosa; M C Irigoyen; M I Phillips; E M Oliveira
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't     Date:  2011-03-29
Journal Detail:
Title:  Physiological genomics     Volume:  43     ISSN:  1531-2267     ISO Abbreviation:  Physiol. Genomics     Publication Date:  2011 Jun 
Date Detail:
Created Date:  2011-06-16     Completed Date:  2011-10-11     Revised Date:  2013-06-30    
Medline Journal Info:
Nlm Unique ID:  9815683     Medline TA:  Physiol Genomics     Country:  United States    
Other Details:
Languages:  eng     Pagination:  665-73     Citation Subset:  IM    
Laboratory of Biochemistry of the Motor Activity, School of Physical Education and Sport, University of Sau Paulo, Sau Paulo, Brazil.
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MeSH Terms
Blood Pressure / drug effects
Cardiomegaly / pathology
Citrate (si)-Synthase / metabolism
Genetic Markers / physiology
Heart Ventricles / metabolism*,  pathology
Hydroxyproline / metabolism
MicroRNAs / metabolism*
Myocytes, Cardiac / pathology
Physical Conditioning, Animal*
Rats, Wistar
Ventricular Function, Left / physiology
Grant Support
1 R01 HL-077602/HL/NHLBI NIH HHS
Reg. No./Substance:
0/Genetic Markers; 0/MIRN29 microRNA, rat; 0/MicroRNAs; 51-35-4/Hydroxyproline; EC (si)-Synthase

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