| MicroRNAs 29 are involved in the improvement of ventricular compliance promoted by aerobic exercise training in rats. | |
| | |
MedLine Citation:
|
PMID: 21447748 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
|
MiRNAs regulate cardiac development, hypertrophy and angiogenesis, but their role in cardiac hypertrophy (CH) induced by aerobic training has not previously been studied. Aerobic training promotes physiological CH preserving cardiac function (CF). This study assessed involvement of miRNAs-29 in CH of trained rats. Female Wistar rats (n=7/group) were randomized into three groups: sedentary (S), training 1 (T1), training 2 (T2). T1: swimming sessions of 60min/5days/week/10weeks. T2: Similar to T1 until 8(th) week. On the 9(th) week rats swam 2x/day, and on the 10th week, 3x/day. MiRNAs analysis was performed by miRNA microarray and confirmed by real-time PCR. We assessed: markers of training, CH by ratio of left ventricle (LV) weight/body weight and cardiomyocytes diameter, pathological markers of CH (ANF, skeletal α-actin, α/β-MHC), collagen I and III (COLIAI and COLIIIAI) by real-time PCR, protein collagen by hydroxyproline (OH-proline) concentration, CF and CH by ecocardiography. Training improved aerobic capacity and induced CH. MiRNAs-1, 133a and 133b were down regulated as observed in pathological CH, however without pathological markers. MiRNA-29c expression increased in T1 (52%) and T2 (123%), correlated with a decrease in COLIAI and COLIIIAI expression in T1 (27%, 38%) and T2 (33%, 48%), respectively. MiRNA-29c was inversely correlated to OH-proline concentration (r=0.61; P<0.05). The E/A ratio increased in T2, indicating improved LV compliance. Thus, these results show that aerobic training increase miR-29 expression and decreased collagen gene expression and concentration in the heart, which is relevant to the improved LV compliance and beneficial cardiac effects, associated with aerobic high performance training. |
| | |
Authors:
|
Ursula Paula Reno Soci; Tiago Fernandes; Nara Yumi Hashimoto; Gloria F Mota; Marco Aurélio Amadeu; Kaleizu Teodoro Rosa; Maria Claudia Irigoyen; M Ian Phillips; Edilamar Menezes Oliveira |
Related Documents
:
|
16874588 - Genetic markers of fibrinolytic responses of older persons to exercise training. 19822938 - The comparison of cortical activation patterns by active exercise, proprioceptive input... 11712588 - Estimating the contributions of mobile sources of pah to urban air using real-time pah ... 11018578 - Exercise adherence among older adults: challenges and strategies. 1596208 - Regional 31p magnetic resonance spectroscopy of exercising human masseter muscle. 6507908 - Differences in threshold for protamine toxicity in isolated atrial and ventricular tissue. |
Publication Detail:
|
Type: JOURNAL ARTICLE Date: 2011-3-29 |
Journal Detail:
|
Title: Physiological genomics Volume: - ISSN: 1531-2267 ISO Abbreviation: - Publication Date: 2011 Mar |
Date Detail:
|
Created Date: 2011-3-30 Completed Date: - Revised Date: - |
Medline Journal Info:
|
Nlm Unique ID: 9815683 Medline TA: Physiol Genomics Country: - |
Other Details:
|
Languages: ENG Pagination: - Citation Subset: - |
Affiliation:
|
School of Physical Education and Sports, University of São Paulo. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
|
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: REGULATION OF WHOLE-BODY ENERGY HOMEOSTASIS WITH GROWTH HORMONE REPLACEMENT THERAPY AND ENDURANCE EX...
Next Document: The Systematic Development of ROsafe: An Intervention to Promote STI Testing Among Vocational School...