Document Detail


MicroRNAome of Porcine Conceptuses and Trophoblasts: Expression Profile of microRNAs and Their Potential to Regulate Genes Crucial for Establishment of Pregnancy.
MedLine Citation:
PMID:  25472924     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
Tightly coordinated, reciprocal embryo-maternal interactions affect gene expression during early pregnancy. Recently, microRNAs (miRNAs) have emerged as new players in the fine tuning of embryo development and implantation in mammals via posttranscriptional gene regulation mechanisms. Here, we integrated transcriptomic and computational approaches to profile miRNAs and miRNA synthesis/transport-related genes during different developmental stages of porcine conceptuses and trophoblast during early pregnancy in the pig. Using quantitative RT-PCR we examined mRNA levels of ten genes encoding proteins involved in miRNA synthesis and transport: DROSHA, DGCR8, XPO5, DICER1, TARBP2, TNRC6A, AGO1, AGO2, AGO3, and AGO4. Custom, multispecies microarrays were used to profile miRNAs. Prediction algorithms of miRNA-mRNA interactions allowed identification of target transcripts for the analyzed miRNAs. These included VEGF, LIF, PTGS2 and IL-6R, known to be crucial components of embryo-maternal interactions in the pig. Two selected miRNAs, miR-26a and miR-125b, were tested for the presence in the extracellular vesicles isolated from uterine luminal flushings during pregnancy. Results of in vitro study demonstrated that miRNAs, such as miR-125b, can regulate expression of genes crucial for embryo development and implantation in porcine endometrial luminal epithelial cells. For the first time, expression profiles of miRNAs and related genes in porcine conceptuses and trophoblast during maternal recognition of pregnancy and embryo implantation in the pig were described. Altogether, our results indicate potential roles of these small, non-coding RNAs in the early development of embryos and embryo-maternal crosstalk during early pregnancy in the pig.
Authors:
Kamil Krawczynski; Joanna Najmula; Stefan Bauersachs; Monika M Kaczmarek
Related Documents :
7279544 - The poggendorff illusion: an illusion of linear extent?
25377504 - Single fetal demise in monochorionic pregnancies: incidence and patterns of cerebral in...
20188484 - Cleft lip and palate: an adverse pregnancy outcome due to undiagnosed maternal and pate...
16129714 - Weight change in monozygotic twins treated with valproate.
7827444 - Subzonal insemination (suzi) or in vitro fertilization (ivf) in microdroplets for the t...
3463214 - Hydatidiform mole: cytogenetic marker analysis in twin gestation. report of two cases.
Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2014-12-3
Journal Detail:
Title:  Biology of reproduction     Volume:  -     ISSN:  1529-7268     ISO Abbreviation:  Biol. Reprod.     Publication Date:  2014 Dec 
Date Detail:
Created Date:  2014-12-4     Completed Date:  -     Revised Date:  2014-12-5    
Medline Journal Info:
Nlm Unique ID:  0207224     Medline TA:  Biol Reprod     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Copyright Information:
Copyright 2014 by The Society for the Study of Reproduction.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Chlamydia muridarum Infection-Induced Destruction of Male Germ Cells and Sertoli Cells Is Partially ...
Next Document:  Predictive value of urine interleukin-18 in the evolution and outcome of acute kidney injury in crit...