Document Detail


MicroRNA (miRNA)-494 regulates mitochondrial biogenesis in skeletal muscle through mitochondrial transcriptional factor A (mtTFA) and forkhead box j3 (Foxj3).
MedLine Citation:
PMID:  23047984     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
MicroRNAs (miRNAs) are important post-transcriptional regulators of various biological pathways. In this study, we focused on the role of miRNAs during mitochondrial biogenesis in skeletal muscle. The expression of miR-494 was markedly decreased in murine myoblast C2C12 cells during myogenic differentiation, accompanied by an increase in mitochondrial DNA. Furthermore, the expression of predicted target genes for miR-494, including mitochondrial transcriptional factor A (mtTFA) and Forkhead box j3 (Foxj3), was post-transcriptionally increased during myogenic differentiation. Knockdown of miR-494 resulted in increased mitochondrial content and upregulation of mtTFA and Foxj3 at the protein level. A 3'UTR reporter assay revealed that miR-494 knockdown directly upregulated the luciferase activity of mtTFA and Foxj3. All of these observations were reversed by overexpression of miR-494. Furthermore, the miR-494 content significantly decreased after endurance exercise in C57BL/6J mice, accompanied by an increase in expression of mtTFA and Foxj3 proteins. These results suggest that miR-494 regulates mitochondrial biogenesis by downregulating mtTFA and Foxj3 during myocyte differentiation and skeletal muscle adaptation to physical exercise.
Authors:
Hirotaka Yamamoyo; Katsutaro Morino; Yoshihiko Nishio; Satishi Ugi; Takeshi Yoshizaki; Atsunori Kashiwagi; Hiroshi Maegawa
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Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2012-10-9
Journal Detail:
Title:  American journal of physiology. Endocrinology and metabolism     Volume:  -     ISSN:  1522-1555     ISO Abbreviation:  Am. J. Physiol. Endocrinol. Metab.     Publication Date:  2012 Oct 
Date Detail:
Created Date:  2012-10-10     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  100901226     Medline TA:  Am J Physiol Endocrinol Metab     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Affiliation:
1Shiga University Of Medical Science.
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