| MicroRNA expression in mouse oligodendrocytes and regulation of proteolipid protein gene expression. | |
| | |
MedLine Citation:
|
PMID: 22504928 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
Overexpression of the major myelin proteolipid protein (PLP) is detrimental to brain development and function and is the most common cause of Pelizaeus-Merzbacher disease. microRNA (miRNA), small, noncoding RNAs, have been shown to play critical roles in oligodendrocyte lineage. In this study, we sought to investigate whether miRNAs control PLP abundance. To identify candidate miRNAs involved in this regulation, we have examined differentiation-induced changes in the expression of miRNAs in the oligodendroglial cell line Oli-neu and in enhanced green fluorescent protein positive oligodendrocytes ex vivo. We have identified 145 miRNAs that are expressed in oligodendrocyte cell lineage progression. Dicer1 expression decreases in differentiated oligodendrocytes, and knock down of Dicer1 results in changes in miRNAs similar to those associated with differentiation. To identify miRNAs that control the PLP expression, we have selected miRNAs whose expression is lower in differentiated vs. undifferentiated Oli-neu cells and that have one or more binding site(s) in the PLP 3'-untranslated region (3'UTR). The PLP 3'UTR fused to the luciferase gene reduces the activity of the reporter, suggesting that it negatively regulates message stability or translation. Such suppression is relieved by knock down of miR-20a. Overexpression of miR-20a decreases expression of the endogenous PLP in primary oligodendrocytes and of the reporter gene. Deletion or mutation of the putative binding site for miR-20a in the PLP 3'UTR abrogated such effects. Our data indicate that miRNA expression is regulated by Dicer1 levels in differentiated oligodendrocytes and that miR-20a, a component of the cluster that controls oligodendrocyte cell number, regulates PLP gene expression through its 3'UTR. |
| | |
Authors:
|
Erming Wang; Franca Cambi |
Related Documents
:
|
7548828 - Vsenod5, vsenod12 and vsenod40 expression during rhizobium-induced nodule formation on ... 17987258 - P-chlorophenoxyisobutyric acid impairs auxin response for gravity-regulated peg formati... 22496868 - Musashi2 is required for the self-renewal and pluripotency of embryonic stem cells. 22395608 - Runx1-induced silencing of non-muscle myosin heavy chain iib contributes to megakaryocy... 9202038 - Transcriptional regulation by triiodothyronine of the udp-glucuronosyltransferase famil... 21068058 - Ongoing roles of phox2 homeodomain transcription factors during neuronal differentiation. |
Publication Detail:
|
Type: Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't Date: 2012-04-14 |
Journal Detail:
|
Title: Journal of neuroscience research Volume: 90 ISSN: 1097-4547 ISO Abbreviation: J. Neurosci. Res. Publication Date: 2012 Sep |
Date Detail:
|
Created Date: 2012-07-11 Completed Date: 2012-11-19 Revised Date: 2013-04-16 |
Medline Journal Info:
|
Nlm Unique ID: 7600111 Medline TA: J Neurosci Res Country: United States |
Other Details:
|
Languages: eng Pagination: 1701-12 Citation Subset: IM |
Copyright Information:
|
Copyright © 2012 Wiley Periodicals, Inc. |
Affiliation:
|
Department of Neurology, University of Kentucky, Lexington, Kentucky, USA. ewang2@uky.edu |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Animals Blotting, Western Cell Differentiation / genetics Cell Separation DEAD-box RNA Helicases / genetics*, metabolism Gene Expression Regulation / genetics* Mice MicroRNAs / genetics*, metabolism Myelin Proteolipid Protein / biosynthesis*, genetics Oligodendroglia / cytology, metabolism* Polymerase Chain Reaction Ribonuclease III / genetics*, metabolism Transcriptome Transfection |
| Grant Support | |
ID/Acronym/Agency:
|
R01 NS053905-04/NS/NINDS NIH HHS; R01NS053905/NS/NINDS NIH HHS |
| Chemical | |
Reg. No./Substance:
|
0/MicroRNAs; 0/Mirn20 microRNA, mouse; 0/Myelin Proteolipid Protein; EC 3.1.26.3/Dicer1 protein, mouse; EC 3.1.26.3/Ribonuclease III; EC 3.6.1.-/DEAD-box RNA Helicases |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Allogeneic stem cell transplantation for elderly patients with myelodysplastic syndrome.
Next Document: Establishment and characterization of a novel chordoma cell line: CH22.