| MicroRNA expression in chicken embryos. | |
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MedLine Citation:
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PMID: 18931185 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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MicroRNA (miRNA) are small single-stranded noncoding RNA that posttranscriptionally regulate gene expression. A major role of miRNA is the regulation of gene expression in developmental processes. In this study, we constructed a small RNA library from 11-d-old chick embryos and used this library to examine the miRNA expression profile of the embryos. This small RNA library was sequenced by using 454 Life Sciences pyrosequencing technology. A total of 10,466 sequences were obtained and annotated as either known chicken miRNA, miRNA that shared homology with other species, or novel miRNA. We identified the expression of 110 known chicken miRNA, 36 homologous chicken miRNA (previously unannotated in the chicken but conserved with miRNA from other species), and 14 novel chicken-specific miRNA not identified in any other species. We also demonstrated that some of the identified chicken embryonic miRNA are differentially expressed among the developing spleen, liver, or bursa. The current study demonstrates that a very diverse and dynamic set of miRNA is expressed in the embryonic chick at 11 d of incubation. The identification of miRNA present in the embryonic chicken will further aid in understanding the complexity of gene regulation during vertebrate development. |
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Authors:
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J A Hicks; P Tembhurne; H-C Liu |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Poultry science Volume: 87 ISSN: 0032-5791 ISO Abbreviation: Poult. Sci. Publication Date: 2008 Nov |
Date Detail:
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Created Date: 2008-10-20 Completed Date: 2008-12-31 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 0401150 Medline TA: Poult Sci Country: United States |
Other Details:
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Languages: eng Pagination: 2335-43 Citation Subset: IM |
Affiliation:
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North Carolina State University, Department of Animal Science, 232D Polk Hall, Campus Box 7621, Raleigh 27695, USA. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Chick Embryo Chickens / genetics* Chromosome Mapping Conserved Sequence DNA Primers Gene Expression Regulation, Developmental Liver MicroRNAs / genetics* Polymerase Chain Reaction Sequence Alignment Sequence Homology, Nucleic Acid Species Specificity Spleen |
| Chemical | |
Reg. No./Substance:
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0/DNA Primers; 0/MicroRNAs |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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