Document Detail


MicroRNA analysis of microdissected normal squamous esophageal epithelium and tumor cells.
MedLine Citation:
PMID:  21796275     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
Previous studies have identified several dysregulated microRNAs in esophageal squamous cell carcinoma (ESCC); however, to date there are no ex vivo analyses comparing expression levels of these regulatory molecules in esophageal squamous cell tumors versus patient-matched normal epithelium. We describe here a technical strategy to evaluate microRNAs in normal esophageal basal cells (NB), normal esophageal differentiated cells (ND), and tumor cells (T). Laser capture microdissection was used to procure target populations from five cases and 18 ESCC-associated microRNAs were measured by RT-qPCR. Five microRNAs (miR-25, miR-106b, miR-21, miR-203, and miR-145) demonstrated consistent differential expression in at least one of the three comparisons: T vs. NB, T vs. ND, or NB vs. ND. The potential regulatory role of the microRNAs in ESCC was further evaluated by correlating their expression with a matched mRNA dataset, which included the same five cases and cell populations. In conclusion, the present work demonstrates the feasibility of studying microRNA levels in precisely dissected cell populations from clinical samples, and sheds light on the molecular mechanisms associated with ESCC.
Authors:
Liang Zhu; Wusheng Yan; Jaime Rodriguez-Canales; Alex M Rosenberg; Nan Hu; Alisa M Goldstein; Philip R Taylor; Heidi S Erickson; Michael R Emmert-Buck; Michael A Tangrea
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Publication Detail:
Type:  JOURNAL ARTICLE    
Journal Detail:
Title:  American journal of cancer research     Volume:  1     ISSN:  2156-6976     ISO Abbreviation:  -     Publication Date:  2011  
Date Detail:
Created Date:  2011-7-28     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  101549944     Medline TA:  Am J Cancer Res     Country:  -    
Other Details:
Languages:  ENG     Pagination:  574-584     Citation Subset:  -    
Affiliation:
Pathogenetics Unit, Laboratory of Pathology, Center for Cancer Research, Bethesda, MD, USA.
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MeSH Terms
Descriptor/Qualifier:
Grant Support
ID/Acronym/Agency:
Z01 SC010437-07//NCI NIH HHS

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