| The microRNA-processing enzyme dicer maintains juxtaglomerular cells. | |
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MedLine Citation:
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PMID: 20056748 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Juxtaglomerular cells are highly specialized myoepithelioid granulated cells located in the glomerular afferent arterioles. These cells synthesize and release renin, which distinguishes them from other cells. How these cells maintain their identity, restricted localization, and fate is unknown and is fundamental to the control of BP and homeostasis of fluid and electrolytes. Because microRNAs may control cell fate via temporal and spatial gene regulation, we generated mice with a conditional deletion of Dicer, the RNase III endonuclease that produces mature microRNAs in cells of the renin lineage. Deletion of Dicer severely reduced the number of juxtaglomerular cells, decreased expression of the renin genes (Ren1 and Ren2), lowered plasma renin concentration, and decreased BP. As a consequence of the disappearance of renin-producing cells, the kidneys developed striking vascular abnormalities and prominent striped fibrosis. We conclude that microRNAs maintain the renin-producing juxtaglomerular cells and the morphologic integrity and function of the kidney. |
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Authors:
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Maria Luisa S Sequeira-Lopez; Eric T Weatherford; Giulianna R Borges; Maria C Monteagudo; Ellen S Pentz; Brian D Harfe; Oscar Carretero; Curt D Sigmund; R Ariel Gomez |
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Publication Detail:
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Type: Journal Article; Research Support, N.I.H., Extramural Date: 2010-01-07 |
Journal Detail:
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Title: Journal of the American Society of Nephrology : JASN Volume: 21 ISSN: 1533-3450 ISO Abbreviation: J. Am. Soc. Nephrol. Publication Date: 2010 Mar |
Date Detail:
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Created Date: 2010-03-01 Completed Date: 2010-03-25 Revised Date: 2011-07-25 |
Medline Journal Info:
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Nlm Unique ID: 9013836 Medline TA: J Am Soc Nephrol Country: United States |
Other Details:
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Languages: eng Pagination: 460-7 Citation Subset: IM |
Affiliation:
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Department of Pediatrics, University of Virginia School of Medicine, Charlottesville, VA 22908, USA. msl7u@virginia.edu |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Blood Pressure / physiology Cell Count DEAD-box RNA Helicases / genetics*, metabolism* Disease Models, Animal Endoribonucleases / genetics*, metabolism* Fibrosis In Situ Hybridization Juxtaglomerular Apparatus* / abnormalities, enzymology, pathology Kidney Diseases / genetics*, metabolism, pathology Mice Mice, Knockout MicroRNAs / metabolism* Renal Circulation / physiology Renin / blood* |
| Grant Support | |
ID/Acronym/Agency:
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DK75481/DK/NIDDK NIH HHS; HL066242/HL/NHLBI NIH HHS; HL48058/HL/NHLBI NIH HHS; HL84207/HL/NHLBI NIH HHS; K08 DK075481-04/DK/NIDDK NIH HHS; K08 DK075481-05/DK/NIDDK NIH HHS; R01 HL096735-02/HL/NHLBI NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/MIRN145 microRNA, mouse; 0/MicroRNAs; EC 3.1.-/Endoribonucleases; EC 3.1.26.3/Dicer1 protein, mouse; EC 3.4.23.15/Renin; EC 3.6.1.-/DEAD-box RNA Helicases |
| Comments/Corrections | |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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