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MicroRNA Expression Patterns Associated with Hyperfunctioning and Non-Hyperfunctioning Phenotypes in Adrenocortical Adenomas.
MedLine Citation:
PMID:  24446485     Owner:  NLM     Status:  Publisher    
BACKGROUND: The adrenocortical adenoma (ACA) entity includes aldosterone-producing (APA), cortisol-producing (CPA) and non-hyperfunctioning (NHFA) phenotypes. While gene mutations and mRNA expression profiles have been partly characterized, less is known about alterations involving microRNA (miRNA) expression.
AIM: To characterize miRNA expression profile in relation to the subtypes of ACAs.
MATERIAL AND METHODS: miRNA expression profiles were determined in 26 ACAs (9 APAs, 10 CPAs, 7 NHFAs) and 4 adrenal references using microarray based screening. Significance analysis of microarrays (SAM) was performed to identify differentially expressed miRNAs between ACA and adrenal cortices, or between tumor subtypes. Selected identified differentially expressed miRNAs were validated in an extended series of 43 ACAs and 10 adrenal references by quantitative RT-PCR.
RESULTS: Hierarchical clustering revealed separate clusters for APAs and CPAs, while the NHFAs were found spread out within the APA/CPA clusters. When NHFA was excluded, the clustering analysis showed a better separation between APA and CPA. SAM analysis identified 40 over-expressed and 3 under-expressed miRNAs in adenomas as compared to adrenal references. Fourteen miRNAs were common among the three ACA subtypes. Furthermore, we found specific miRNAs associated with different tumor phenotypes.
CONCLUSION: The results suggest that miRNA expression profiles can distinguish different subtypes of ACA, which may contribute to a deeper understanding of ACA development and potential therapeutics.
David Velazquez-Fernandez; Stefano Caramuta; Deniz M Ozata; Ming Lu; Anders Höög; Martin Bäckdahl; Catharina Larsson; Weng-Onn Lui; Jan Zedenius
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Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2014-1-20
Journal Detail:
Title:  European journal of endocrinology / European Federation of Endocrine Societies     Volume:  -     ISSN:  1479-683X     ISO Abbreviation:  Eur. J. Endocrinol.     Publication Date:  2014 Jan 
Date Detail:
Created Date:  2014-1-21     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9423848     Medline TA:  Eur J Endocrinol     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
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