Document Detail


MicroRNA-375 targets PDK1 in pancreatic carcinoma and suppresses cell growth through the Akt signaling pathway.
MedLine Citation:
PMID:  24481267     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
MicroRNAs (miRNAs or miRs) are believed to have great potential for use as molecular targets in the diagnosis and treatment of cancer. In this study, we demonstrate that miR-375 is downregulated in pancreatic carcinoma (PC) tissues and PC cell lines. We found that miR-375 negatively regulates the expression of 3-phosphoinositide-dependent protein kinase 1 (PDK1) by directly targeting the 3'UTR of the PDK1 transcript. To investigate the biological roles and the potential mechanisms of action of miR‑375, we induced either the up- or downregulation of miR-375 expression by transfecting various PC cells with miR-375 mimics or an inhibitor. Our results revealed that the upregulation of miR-375 inhibited cell growth and induced cell apoptosis, while the downregulation of miR-375 with the inhibitor had the opposite effect. In addition, our data demonstrate that miR-375 suppresses the malignant behavior of PC cells through the Akt signaling pathway rather than mitogen-activated protein kinase (MAPK) signaling pathways. Taken together, our findings indicate that targeting miR-375 by a genetic approach may provide a novel strategy for the treatment of PC.
Authors:
Jian Zhou; Shiduo Song; Songbing He; Xinguo Zhu; Yi Zhang; Bin Yi; Bing Zhang; Gongzhao Qin; Dechun Li
Related Documents :
23856837 - P38δ mitogen-activated protein kinase regulates the expression of tight junction protei...
11867627 - C-src mediates neurite outgrowth through recruitment of crk to the scaffolding protein ...
14522827 - Pyrazolo pyrimidine-type inhibitors of src family tyrosine kinases promote ovarian ster...
10477727 - Signaling via src family kinases is required for normal internalization of the receptor...
24810047 - Identification of prolidase as a high affinity ligand of the erbb2 receptor and its reg...
17875337 - An efficient method for protein phosphorylation using the artificially introduced of co...
Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2014-1-28
Journal Detail:
Title:  International journal of molecular medicine     Volume:  -     ISSN:  1791-244X     ISO Abbreviation:  Int. J. Mol. Med.     Publication Date:  2014 Jan 
Date Detail:
Created Date:  2014-1-31     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9810955     Medline TA:  Int J Mol Med     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  LXR Agonist Regulates the Carcinogenesis of PCa via the SOCS3 Pathway.
Next Document:  Chorioamnionitis as a Risk Factor for Retinopathy of Prematurity: A Systematic Review and Meta-Analy...