Document Detail

MicroRNA-375 targets PDK1 in pancreatic carcinoma and suppresses cell growth through the Akt signaling pathway.
MedLine Citation:
PMID:  24481267     Owner:  NLM     Status:  Publisher    
MicroRNAs (miRNAs or miRs) are believed to have great potential for use as molecular targets in the diagnosis and treatment of cancer. In this study, we demonstrate that miR-375 is downregulated in pancreatic carcinoma (PC) tissues and PC cell lines. We found that miR-375 negatively regulates the expression of 3-phosphoinositide-dependent protein kinase 1 (PDK1) by directly targeting the 3'UTR of the PDK1 transcript. To investigate the biological roles and the potential mechanisms of action of miR‑375, we induced either the up- or downregulation of miR-375 expression by transfecting various PC cells with miR-375 mimics or an inhibitor. Our results revealed that the upregulation of miR-375 inhibited cell growth and induced cell apoptosis, while the downregulation of miR-375 with the inhibitor had the opposite effect. In addition, our data demonstrate that miR-375 suppresses the malignant behavior of PC cells through the Akt signaling pathway rather than mitogen-activated protein kinase (MAPK) signaling pathways. Taken together, our findings indicate that targeting miR-375 by a genetic approach may provide a novel strategy for the treatment of PC.
Jian Zhou; Shiduo Song; Songbing He; Xinguo Zhu; Yi Zhang; Bin Yi; Bing Zhang; Gongzhao Qin; Dechun Li
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Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2014-1-28
Journal Detail:
Title:  International journal of molecular medicine     Volume:  -     ISSN:  1791-244X     ISO Abbreviation:  Int. J. Mol. Med.     Publication Date:  2014 Jan 
Date Detail:
Created Date:  2014-1-31     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9810955     Medline TA:  Int J Mol Med     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
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