| MicroRNA-20a Overexpression Inhibited Proliferation and Metastasis of Pancreatic Carcinoma Cells. | |
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MedLine Citation:
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PMID: 20583868 Owner: NLM Status: In-Data-Review |
Abstract/OtherAbstract:
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Abstract The aim of this study was to investigate the effect of microRNA-20a on pancreatic carcinoma cell proliferation and invasion and to find a new effective treatment strategy for pancreatic carcinoma. MicroRNA-20a expression was determined in 10 matched normal pancreatic tissues and pancreatic carcinoma by in situ hybridization. Quantitative real-time RT-PCR was used to evaluate the expression of microRNA-20a in two pancreatic carcinoma cell lines (BxPC-3 and Panc-1) and immortal human pancreatic duct epithelial cell line H6C7. Proliferation and invasion capacity were analyzed for the cells with lentivirus-mediated overexpression of microRNA-20a both in vitro and in vivo. In addition, the regulation of signal transducer and activator of transcription proteins 3 (Stat3) by microRNA-20a was determined to elucidate the underlying mechanisms. The pancreatic cancer cell lines (Panc-1 and BxPC-3) stably overexpressing microRNA-20a showed reduced proliferation and invasion capacity in vitro and in vivo, compared with parental cells or cells transfected with a control vector. Furthermore, we found that microRNA-20a negatively regulated Stat3 protein expression in a dose-dependent manner without changing the Stat3 mRNA level and decreased the activity of a luciferase reporter construct containing the Stat3 3'-untranslated region. These results show that microRNA-20a regulates Stat3 at the post-transcriptional level, resulting in inhibition of cell proliferation and invasion of pancreatic carcinoma. It may open a new perspective for the development of effective gene therapy for pancreatic carcinoma. |
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Authors:
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Haijiao Yan; Jiangxue Wu; Wensong Liu; Yufang Zuo; Shupeng Chen; Shineng Zhang; Musheng Zeng; Wenlin Huang |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Human gene therapy Volume: 21 ISSN: 1557-7422 ISO Abbreviation: Hum. Gene Ther. Publication Date: 2010 Dec |
Date Detail:
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Created Date: 2010-12-14 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 9008950 Medline TA: Hum Gene Ther Country: United States |
Other Details:
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Languages: eng Pagination: 1723-34 Citation Subset: IM |
Affiliation:
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1 State Key Laboratory of Oncology in South China, Cancer Center, Sun Yat-sen University , Guangzhou 510060, P.R. China . |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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