Document Detail


MicroRNA-204 Regulates Vascular Smooth Muscle Cell Calcification in vitro and in vivo.
MedLine Citation:
PMID:  22871591     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
AIMS: Medial artery calcification is a common macroangiopathy that initiates from a cell-regulated process similar to osteogenesis. Although the mechanisms governing this process remain unclear, epigenomic regulation by specific microRNAs might play a role in vascular smooth muscle cell (VSMC) calcification. In this study, we aimed to investigate whether miR-204 participates in regulating VSMC calcification. METHODS AND RESULTS: We found that miR-204 was suppressed in mouse aortic VSMCs during β-glycerophosphate induced calcification, whereas Runx2 protein levels were elevated. Overexpression of miR-204 by transfection of miR-204 mimics decreased Runx2 protein levels and alleviated β-glycerophosphate induced osteoblastic differentiation of VSMCs while miR-204 inhibition by transfection of miR-204 inhibitors significantly elevated Runx2 protein levels and enhanced osteoblastic differentiation of VSMCs, suggesting miR-204's role as an endogenous attenuator of Runx2 in VSMC calcification. Luciferase reporter assays revealed Runx2 as the direct target of miR-204 by overexpression of miR-204 on wild-type or mutant 3'-UTR sequences of Runx2 in VSMCs. In vivo overexpression of miR-204 by injection of miR-204 agomirs in Kunming mice attenuated vitamin D3 induced medial artery calcification. CONCLUSION: Our study show that down-regulation of miR-204 may contribute to β-glycerophosphate induced VSMC calcification through regulating Runx2. miR-204 represents an important new regulator of VSMC calcification and a potential therapeutic target in medial artery calcification.
Authors:
Rong-Rong Cui; Shi-Jun Li; Ling-Juan Liu; Lu Yi; Qiu-Hua Liang; Xiao Zhu; Guan-Ying Liu; Yuan Liu; Shan-Shan Wu; Xiao-Bo Liao; Ling-Qing Yuan; Ding-An Mao; Er-Yuan Liao
Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2012-8-7
Journal Detail:
Title:  Cardiovascular research     Volume:  -     ISSN:  1755-3245     ISO Abbreviation:  Cardiovasc. Res.     Publication Date:  2012 Aug 
Date Detail:
Created Date:  2012-8-8     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0077427     Medline TA:  Cardiovasc Res     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Affiliation:
Department of Pediatrics, The Second Xiang-Ya Hospital, Central South University, #139 Middle Renmin Road, 410011, Changsha, Hunan, People's Republic of China.
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